HPLC法测定清喉利咽颗粒中柚皮苷、橙皮苷的含量

目的：建立以HPLC法测定清喉利咽颗粒中柚皮苷、橙皮苷含量的方法。方法：采用高效液相色谱法，色谱柱：Phe—nomenex C18柱（250mm×4．6mm，4μm），流动相：甲醇－水（40：60），流速：1．0ml·min^-1，检测波长：283nm，柱温：30oc。结果：柚皮苷在352—3520ng范围内线性关系良好（r=0．9996），平均回收率为99．53％，RSD=0．39％（n=6）；橙皮苷在204～2040ng范围内线性关系良好（r=0．9992），平均回收率为99．12％，RSD=0．50％（n=6）。结论：该法操作简便，结果准确可靠，可作为清喉利咽颗粒的质量控制方法。

Determination of Naringin, Hesperidin in Qinghou Liyan Granules by HPLC

Objective： To establish an HPLC method for determination of Naringin and Hesperidin in qinghou liyan granules. Method： A Phenomenex Cis Column （250 mm ×4. 6 mm,4μm） was used. The mobile phase was methanol-water （40： 60）. The column temperature was 30℃ ,The waveleng detection were at 283nm,flow rate was 1.0 ml.min^-1. Result： The linear range of Naringin was within 352-3520 ng（r =0. 999 6）. The average recovery was 99. 53% ,RSD =0. 39% （n =6）. The linear range of Hesperidin was within 204-2 040 ng （ r = 0. 999 2 ）. The average recovery was 99. 12% , RSD = 0. 50% （ n = 6 ）. Conclusion： This method is simple, accurate and suitable for its assaying. It can be used for quality control in production of qinghou liyan granules.