气相色谱外标法、内标法测定广藿香中百秋李醇含量的比较

目的：建立气相外标法测定广藿香中百秋李醇的含量。方法：色谱柱为弹性石英毛细管柱ZB-5（30m×0．32mm×0．25μm）；程序升温：初始温度190℃，保持6min，以40℃·min。的速率升温至230℃，保持2min，进样口温度为280℃，检测器温度为280℃；分流为20：1，进样量：1μl。结果：百秋李醇在0．0110～0．5525mg·ml^-1的范围内呈良好线性关系（r=0．9999）；平均回收率为100．1％，RSD=2．0％（n：6）。本试验所建立的外标法测得的13批样品中百秋李醇含量都高于内标法。结论：该方法准确简便、环保、重复性好，较原方法更能准确地测定广藿香中百秋李醇的含量。

Comparison on Methods of Determination the Contents of Patchouli Alcohol in Herba Pogostemonis by GC-ESTD and GC-ISTD

Objective： To establish a external standard method for determination of the patchouli alcohol in herba pogostemonis. Method： The ZB-5 column （30 m × 0. 32 mm × 0. 25μm）was used. Temperature was programmed：initial temperature was 190 ℃, and maintained around 6 minutes, then heated to 230 ℃ at rate of 40 ℃ .min^-1, maintained 2 min. The inlet temperature was 280 ℃, detector temperature was 280 ℃ ,split ratio was 20：1 ,and injection volume was 1 μl. Result： Good linearity of patchouli alcohol was within the range of 0. 011 0-0. 552 5 mg.ml^-1 （ r = 0. 999 9）. The average recovery was 100. 1% , and RSD was 2. 0% （ n = 6 ）. Conclusion： The method is simple, accurate, environmental, repeatable and more accurate than the original method of determination of patchouli alcohol in herba pogostemonis