HPLC法同时测定养血化瘀合剂中阿魏酸和甘草酸的含量

目的：建立HPLC法同时测定养血化瘀合剂中阿魏酸、甘草酸含量的方法。方法：采用Diamonsil C18（200mm×4．6mm，5μm）色谱柱，以乙腈为流动相A，0．085％磷酸水溶液为流动相B，进行线性梯度洗脱，流速为1．0ml·min^-1，检测波长为245nm，柱温为25℃。结果：阿魏酸在5．02～50．24μg·ml^-1（r=0．9．999）和甘草酸在2．46～24．62μg·ml^-1（r=0．9999）的范围内浓度与峰面积呈良好的线性关系，阿魏酸和甘草酸的加样回收率分别为100．1％（n=9，RSD=1．57％）和99．8％（n=9，RSD=0．98％）。结论：本法操作简单、快速、重复性好，结果准确可靠，可用于养血化瘀合剂的质量控制。

Simultaneous Determination of Ferulic Acid and Glycyrrhizic Acid in Yangxuehuayu Mixture by HPLC

Objective： To establish an HPLC method for the simultaneous determination of ferulic acid and glycyrrhizic acid in yangxuehuayu mixture. Method： The Diamonsil C18 （200 mm ×4. 6 mm,5 ℃m）column was used. The mobile phase was acetonitrile and 0. 085 % phosphoric acid with gradient elution. The flow rate was 1 ml. min -1. The wavelength was 245 nm. The temperature of column was 25 ℃. Result： The linear ranges of ferulic acid and glycyrrhizic acid were 5.02-50. 24 μg·ml-1 （ r = 0. 999 9 ） and 2.46- 24. 62 μg· ml - 1 （ r = 0. 999 9 ）. The average recoveries were 100. 1% （ n = 9, RSD = 1.57% ） and 99. 8% （ n = 9, RSD = 0. 98% ）, respectively. Conclusion： The method is simple, reliable, and reproducible, it can be used to control the quality of yangxuehuayu mixture.