多囊卵巢综合症患者血浆PAI-1水平及基因4G5G多态性的研究

目的 研究多囊卵巢综合症（PCOS）纤溶酶原激活物抑制因子-1（PAI-1）基因启动子区4G5G多态性的分布,探讨其与PCOS发病关系.方法 96例PCOS患者和50例对照组,用PCR方法测定PAI-1基因启动子区4G5G基因多态性;ELISA法检测血浆PAI-1水平.结果 PCOS组4G/4G基因型明显高于对照组,而5G/5G基因型显著少于对照组.PCOS组的PAI-1水平较对照组明显升高（29.32±2.37ng/mL与22.42±0.65ng/mL,P〈0.05）.结论 PCOS患者PAI-1基因启动子区4G/4G基因型频率增高,致血浆PAI-1水平升高,其与PCOS的发病有关.

The Study of 4G5G Polymorphism of Plasminogen Activator Inhibitor-1(PAI-1) Gene in Polycystic Ovarian Syndrome and Its Association with Plasma PAI-1 Levels

Objectives To investigate the prevalence of 4G5G polymorphism of plasminogen activator inhibitor-l（PAI-1） gene in polycystic ovary syndrome（PCOS） and its clinical significance. Methods We studied 96 PCOS patients and 50 controls.Blood samples were obtained for DNA analysis.Plasma PAI-I levels were measured by the ELISA in all subjects.The 4G5G polymorphic site of the PAI-I gene promoter region was amplified by the polymerase chain reaction（PCR）. Results The PCOS group had significantly higher 4G/4G than that of the control group,whereas 5g/5G were less significant.The group of PCOS had significantly higher PAI-1 levels than that of the controls（29.32±2.37ng/mL vs 22.42 e 0.65ng/mL,P 〈 0.05）. Conelusions The genotypic subtype 4G/4G of the PCOS was significantly higher than that of the controls.PCOS patients have higher levels of PAl-1 compared with the control group.PAl-1 gene polymorphism 4G genetype may be correlated with PCOS.