HPLC-ELSD测定黄氏响声丸中贝母素甲和贝母素乙的含量

目的：建立黄氏响声丸中贝母素甲和贝母素乙的含量测定方法。方法：采用HPLC法，色谱柱：Agilent ZORBAX SB—C18柱（4．6mm×250mm，5μm），流动相为乙腈-水-二乙胺（65：35：0．03），流速为1．0mL·min^-1，柱温为30℃；SOFTA-300SELSD型蒸发光散射检测器（漂移管温度85℃，雾化室温度50℃）。结果：贝母素甲进样量在0．5056—10．1120μg范围内，贝母素乙进样量在0．4106～8．2120μg范围内，其进样量的自然对数与峰面积积分值的自然对数有良好的线性关系（r〉0．9990），贝母素甲和贝母素乙平均加样回收率分别为97．69％和97．39％，RSD分别为1．61％和1．37％。结论：该方法稳定、重复性好，结果准确，可作为黄氏响声丸中贝母素甲和贝母素乙的含量测定方法。

Determination of Peimine and Peiminine in Huangshi Xiangsheng Pills by HPLC-ELSD

Objective： To establish an HPLC-ELSD method for determing Peimine and Peiminine in Huangshi Xiangsheng Pills. Methods ： Chromatographic conditions included Agilent ZORBAX SB - C18 column （4. 6 mm×250mm,5μm ） and the mobile phase consisting of a mixture of acetonitrile, water and diethylamine （65： 35： 0. 03 ）. The flow rate of mobile phase was 1.0 mL ·min^-1. The column temperature was at 30 ℃. Deteetor：SOFTA-300S ELSD （Eva：85 ℃ , Ned ：50 ℃ ）. Results：The linear ranges of Peimine and Peiminine were 0. 505 6 - 10. 112 0 p,g（r =0. 999 4）and 0. 410 6 -8. 212 0 μg（r =0. 999 2）, respectively. The average recoveries of Peimine and Peiminine were 97. 69% and 97. 39% with corresponding RSD of 1.61% and 1.37% （n = 6） , respectively. Conclusion： The method is steady and with good repeatability, and can be used to determine the content of peimine and peiminine in Huangshi Xiangsheng Pills.