温胆汤对精神分裂症模型鼠血清SOD, MDA,NO,PKC的影响

目的: 研究温胆汤(由半夏、茯苓、竹茹、枳实、陈皮、炙甘草配伍)对精神分裂症模型鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)及蛋白激酶C(PKC)含量的影响。 方法: 将50只清洁级SD大鼠随机分为5组:正常组(A)、模型组(B)、温胆汤高剂量组(C)、温胆汤中剂量组(D)、温胆汤低剂量组(E),每组10只。在造模前,C,D,E组连续ig温胆汤,每次给药20 mL·kg^-1(C,D,E组剂量相当于生药40,20,10 g·kg^-1);A、B组用等量生理盐水ig,1次/d,共21 d。在最后1次给药2 h后,B,C,D,E组一次性左侧腹腔ip MK801 0.6 mg·kg^-1,建立精神分裂症模型;一般状况观察3 d后,处死大鼠,取血清检测。采用生化方法测定血清SOD,MDA和NO含量的改变,用双抗体夹心-酶联免疫吸附(ABC-ELISA)法检测血清中PKC的含量。 结果: 与模型组比较,温胆汤各组均不同程度改善了模型鼠的一般状况,并且明显降低血清MDA,NO的含量(P<0.05或P<0.01),升高SOD的活性(P<0.05或P<0.01),减轻了氧自由基引起的病理损伤。温胆汤各组显著降低血清PKC的含量(P<0.05或P<0.01)。 结论: 温胆汤可减轻精神分裂症模型鼠的病理损害,对氧自由基引起的损伤具有一定的保护;降低PKC浓度,从而对缝隙连接通讯(GJIC)的功能起到调节作用。

Effect of Wendan Decoction on SOD,MDA,NO and PKC in Rats Serum with Schizophrenia

Objective:To research the effect of Wendan decoction[It was made up of Pinelliae Rhizoma, Poria, Bambusae Caulis in Taenias, Aurantii Fructus Immaturus, Citri Reticulatae Pericarpium, Glycyrrhizae Radix et Rhizoma Prasparata Cum Melle.]on superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO) and protein kinase(PKC) in rats serum with schizophrenia. Method:Fifty SD rats were randomly divided five groups: normal group(A), model group(B), Wendan decoction high-dosage group(C), Wendan decoction medium-dosage group(D) and Wendan decoction low-dosage group(E), ten rats in each group. Before established the model, the C, D, E groups were given Wendan decoction (40, 20, 10 g·kg^-1) and the A and B groups were given normal saline every day, which last for 21 days. After two hours at the last given Wendan decoction or saline, the B, C, D, E groups were ip given MK801 0.6 mg·kg^-1 to establish rat schizophrenia the model at the left side of the rat abdominal cavity. After general condition of rats was observed three days, the rats were sacrificed and serum was tested. The superoxide dismutase(SOD), malondialdehyde(MDA), nitri oxide(NO) in rats serum were detected by biochemical methods and the PKC in rats serum was detected by double antibody clip ELASA method. Result:Compared with B group, general condition of rats was improved, the MDA, NO, PKC content of group C, D and E were significantly decreased (P <0.05 or P <0.01) and their SOD was significantly increased (P <0.05 or P <0.01), pathological damage caused by oxygen free radical was alleviated. The PKC content of group C, D and E were significantly decreased (P <0.05 or P <0.01). Conclusion:Wendan decoction could reduce the pathologic lesions of rat with schizophrenia, protect from the damage caused by oxygen free radicals; Wendan decoction could decrease PKC content and thus play a regulatory role in the function of GJIC.