狗肝菜不同相对分子质量多糖对D-GlaN致大鼠急性肝损伤的保护作用

目的:探讨狗肝菜不同相对分子质量多糖对大鼠D-半乳糖胺(D-GlaN)致急性肝损伤的保护作用.方法:采用透析的方法将狗肝菜多糖分为＜6 kD,＞6kD＜10kD(6kD～10kD),＞10 kD,以及总多糖组.D-GlaN (500 mg·kg-1)ip诱导大鼠急性肝损伤模型,动物处死后测定血清中天冬氨酸转氨酶(AST),丙氨酸转氨酶(ALT)活性,制备肝组织匀浆测定其中丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)含量,取肝、脾脏称重,计算脏器指数,对数据进行统计分析,并观察大鼠肝脏组织形态学变化.结果:与模型组比较,狗肝菜总多糖和相对分子质量＜6 kD给药组能显著降低大鼠血清中AST,ALT活性(P＜0.01);各给药组均能降低肝匀浆MDA含量(P＜0.01或P＜0.05),均能有效升高肝组织匀浆中GSH-Px活性(P＜0.01或P＜0.05),降低肝脏指数(P＜0.01或P＜0.05),减轻肝脏病理损伤.结论:狗肝菜各多糖给药组均有一定保肝作用,且以相对分子质量＜6 kD效果最佳.

Protective Effects of Different Molecule Weight Range of Dicliptera chinensis Polysaccharide against D-GlaN-induced Acute Liver Injury in Rats

Objective:This study evaluated the hepatoprotective activity of different molecule weight range of Dicliptera chinensis polysaccharide (DCP) against D-galactosamine (D-GlaN)-induced hepatotoxicity in SD rats. Method: DCP was divided into three parts according to the semipermeable membrane with different molecular weight cutoff (MWCO) using dialysis. Mw<6 kD, Mw 6 kD-10 kD, Mw>10 kD and DCP were studied on D-GlaN-induced (500 mg·kg^-1 intraperitoneally) hepatic damage in rats. Liver coefficient, serum enzyms as alanine aminotransferase(ALT), aspartate aminotransferase(AST), hepatic tissue index malondialdehyde (MDA) and glutathione peroxidase (GSH-Px), and histopathological Changes in liver architecture including necrosis and swelling of hepatic cells were detected. Result: Compared with the model group, pretreated with DCP and Mw<6 kD polysaccharide significantly prevented the increases of alanine ALT and AST level in serum, each group can prevented MDA accumulation, GSH-Px depletion in liver tissue, and hepatocyte swelling and necrosis. Conclusion: The results indicate that DCP can protect against D-GlaN-induced acute liver injury in rats, and the most active part is the range of molecular weight left than 6 kD.