4个Y-STR基因座在海南岛黎族人群的遗传多态分布

目的:调查DYS3891、DYS390、DYS391和DYS393基因座在海南岛黎族群体中的分布状况。方法:应用多重PCR扩增后,用ABI377自动测序仪进行基因扫描分型。结果:在230名无亲缘关系的黎族男性个体中观察到黎族群体在4个基因座均有遗传多态性;DYS3891观察到4种等位基因;DYS390观察到5种等位基因;DYS391观察到4种等位基因;DYS393观察到5种等位基因。获得了黎族群体在4个Y-STR基因位点的等位基因频率、基因多样性和单体多样性数据。结论:4个Y-STR基因位点的单倍型检测在法医学个体识别和亲权鉴定及黎族的起源研究有较高的应用价值。

THE GENETIC POLYMORPHISMS OF FOUR Y-STR LOCUS IN LI ETHNIC GROUPS OF HAINAN ISLAND

Objective:To investigate the distribution of genetic polymorphism of four Y chromosome specific short tandem repeat ( Y-specific STR ) loci in Li ethnic group in Hainan Island. Method:Four tetranucleotide STR loci were simultaneously amplified with fluorescently labeled primers and genotypes were determined with an automated DNA sequencer.Res ults:Among230unrelated males,the alleles at the four Y-specific STR loci were composed of some complex repeat structure.4?5?4?5alleles were observed in locus DYS3891?DYS390?DYS391?DYS393respectively.A set of human allele ladders for the typing of the four Y-specific STRs was obtained in Li ethnic population.Gene diversity index(D)and haplotype diversity data were estimated for the four Y STRs.Conclus ion:The preliminary study indicates a reference population for detecting male migration events and should be useful in population genetic and forensic applications.