| HPLC法测定硫辛酸缓释片中主药的含量 |
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| 引用本文： | 孙靓,张守尧,张忠义,季爱民. HPLC法测定硫辛酸缓释片中主药的含量[J]. 中国药房, 2008, 19(19): 1501-1502 |
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| 作者姓名： | 孙靓 张守尧 张忠义 季爱民 |
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| 作者单位： | 孙靓 (南方医科大学珠江医院药剂科,广州市,510282)； 张守尧 (南方医科大学珠江医院药剂科,广州市,510282)； 张忠义 (南方医科大学珠江医院药剂科,广州市,510282)； 季爱民 (南方医科大学珠江医院药剂科,广州市,510282)； |
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| 摘 要： | 目的:建立以高效液相色谱法测定硫辛酸缓释片中主药含量的方法。方法:色谱柱为HypersilODS,流动相为乙腈-0.05mol·L-1磷酸二氢钾溶液(45∶55,用磷酸调pH为2.0),流速为1.0mL·min-1,进样量为10μL,检测波长为219nm,柱温为25℃。结果:硫辛酸检测浓度的线性范围为9.99～399.70μg·mL-1(r=0.999);平均回收率为99.17%(RSD=0.40%);日内、日间RSD均小于2.6%。结论:本方法简便、准确,可用于硫辛酸的含量测定。
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| 关 键 词： | 高效液相色谱法 硫辛酸缓释片 含量 |
Determination of the Content of Main Components in Lipoic Acid Sustained- release Tablets by HPLC |
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| SUN Liang,ZHANG Shou-yao,ZHANG Zhong-yi,JI Ai-min. Determination of the Content of Main Components in Lipoic Acid Sustained- release Tablets by HPLC[J]. China Pharmacy, 2008, 19(19): 1501-1502 |
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| Authors: | SUN Liang ZHANG Shou-yao ZHANG Zhong-yi JI Ai-min |
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| Affiliation: | (Dept. of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China) |
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| Abstract: | OBJECTIVE： To establish an HPLC method for the determination of lipoic acid in lipoic acid sustained- release tablets. METHODS： HPLC was conducted on a Hypersil ODS with acetonitrile- 0.05 mol · mL^-1 potassium dihydrogen phosphate （45 ： 55, adjust pH to 2.0 ） as mobile phase at a flow rate of 1.0 mL · min ^-1. The injection volume was 10μL; the detection wavelength was set at 219 nm and the column temperature was 25 ℃. RESULTS： The linear range of lipoic acid was 9.99-399.70μg ·mL^-1（ r = 0. 999） with an average recovery rate of 99.17% （RSD = 0.40 % ） ; the intra - day and inter- day RSD were all less than 2.6%. CONCLUSION： The method is simple and accurate, and suitable for the determination of the content of lipoic acid in lipoic acid sustained- release tablets. |
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| Keywords: | HPLC Lipoic acid sustained release tablets Content |
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