通心络对兔缺氧耐受性的影响

目的观察通心络对兔急性缺氧耐受性的作用。方法 24只新西兰大耳白家兔随机分为对照组、缺氧组和通心络组,先在11.4%氧环境下低氧实验,60min后进行密闭缺氧,观察兔密闭缺氧耐受时间,ELISA法检测血清缺氧诱导因子1-α(HIF-1α)的含量、Western blot法检测主动脉组织HIF-1α和血管内皮生长因子(VEGF)的蛋白表达。结果与缺氧前比较,缺氧组与通心络组血清HIF-1α含量明显增加(P<0.01或P<0.05)。与对照组比较,缺氧组和通心络组主动脉组织HIF-1α和VEGF表达明显增强(P<0.01或P<0.05)。与缺氧组比较,通心络组家兔密闭缺氧耐受时间明显增加(P<0.05),血清HIF-1α含量明显增加(P<0.01),主动脉组织HIF-1α表达增强,VEGF表达明显增强(P<0.05)。结论 HIF-1α和VEGF的表达增高可能是缺氧适应的一种机制,通心络能提高兔的缺氧耐受性,其机制可能与上调血清及组织的HIF-1α和VEGF有关。

Effects of Tongxinluo on hypoxia tolerance of rabbit

Aim To investigate the effects of Tongxinluo(TXL,a Chinese medicine) on the hypoxia tolerance of rabbits with acute hypoxia and to explore the mechanism.Methods Twenty-four healthy flap-eared white rabbits were randomly divided into 3 groups:control group,hypoxia group and TXL group.The rabbits were in low oxygen condition of 11.4%O2 at first.After 60 minutes,air supply was closed.The hypoxia tolerance time of the rabbits was measured.The content of serum hypoxia inducible factor 1alpha(HIF-1α) was detected with ELISA.The expression of HIF-1αand vascular endothelial growth factor(VEGF) in the aorta of rabbit were detected by Western blot.Results Compared with hypoxia before,the serum HIF-1αof hypoxia and TXL groups increased significantly(P<0.05 or P< 0.01) .Compared with the control group,the HIF-1α and VEGF in the aorta of hypoxia and TXL groups expressed obviously(P<0.05 or P<0.01) .Compared with hypoxia group,the hypoxia tolerance time of TXL group increased significantly(P<0.01) ,the serum HIF-1αcontent increased significantly at 60 minutes(P <0.01) ,and the expression of HIF-1αand VEGF in aorta tissue was enhanced obviously(P<0.05) .Conclusions These results indicate that hypoxia can induce HIF-1αand VEGF by itself to adaptation.TXL can improve the hypoxia tolerance of rabbits.The impossible mechanism may be related to the up-regulation of HIF-1αand VEGF in serum and aorta tissue.