|
|||||
|
|
| 外用表没食子儿茶素没食子酸酯抵抗中波紫外线对小鼠皮肤的慢性光损伤作用 | |
| 引用本文： | 徐丽贤,骆丹,徐晶,林向飞,吉玺,朱洁,吴迪,李巍.外用表没食子儿茶素没食子酸酯抵抗中波紫外线对小鼠皮肤的慢性光损伤作用[J].临床皮肤科杂志,2008,37(11):695-698. |
| 作者姓名： | 徐丽贤 骆丹 徐晶 林向飞 吉玺 朱洁 吴迪 李巍 |
| 作者单位： | 1. 江苏省省级机关医院皮肤科,210024 2. 南京医科大学第一附属医院皮肤性病科,江苏,南京,210029 3. 扬州大学医学院病理教研室,江苏,扬州,225001 |
| 基金项目： | 国家自然科学基金资助项目(30371294) |
| 摘 要： | 目的：研究中波紫外线（UVB）慢性辐射BALB／C小鼠后皮肤的组织病理改变、表皮细胞凋亡情况及表没食子儿茶素没食子酸酯（EGCG）对其的影响。方法：EGCG局部外用于小鼠耳、背部皮肤后分别给予不同强度的UVB辐射，每日1次．连续1个月，石蜡切片苏木精-伊红染色观察不同处理条件下皮肤组织病理变化，同时应用末端转移酶介导的缺口末端标记（TUNEL）法检测小鼠表皮中的凋亡细胞。结果：UVB慢性辐射对BALB／C小鼠皮肤有明显影响，主要表现有表皮过度角化、棘层肥厚、海绵样水肿、晒斑细胞、真皮乳头层水肿、毛细血管扩张、炎性细胞浸润等。EGCG预处理能减轻UVB诱导的上述组织病理变化。另外，对慢性低剂量UVB辐射组，EGCG有一定的促细胞凋亡作用。结论：不同剂量UVB慢性辐射后小鼠皮肤组织病理改变明显，EGCG可保护UVB辐射诱导的光损伤作用，并对低剂量慢性UVB辐射后BALB／C小鼠皮肤细胞有促进凋亡作用。 |
| 关 键 词： | 表没食子儿茶素没食子酸酯 紫外线 中波 BALB／C小鼠 |
Protective effect of topical(-)-epigallocatechin-3-gallate against chronic photodamage induced by UVB irradiation on the skin of BALB/C mouse |
|
| XU Li-xian,LUO Dan,XU Jing,LIN Xiang-fei,JI Xi,ZHU Jie,WU Di,LI Wei.Protective effect of topical(-)-epigallocatechin-3-gallate against chronic photodamage induced by UVB irradiation on the skin of BALB/C mouse[J].Journal of Clinical Dermatology,2008,37(11):695-698. | |
| Authors: | XU Li-xian LUO Dan XU Jing LIN Xiang-fei JI Xi ZHU Jie WU Di LI Wei |
| Institution: | Department of Dermatology and Venereology;the First Affiliated Hospital of Nanjing Medical University;Nanjing 210029;China |
| Abstract: | Objective:To investigate the pathological changes and the apoptosis of epidermal cells on the skin of BALB/C mouse induced by chronic ultraviolet B(UVB) irradiation and the photoprotective effect of topical application with(-)-epigallocatechin-3-gallate(EGCG) against it. Methods:The skin of the ears and the back of BLAB/C mice was pretreated with EGCG and then irradiated with different doses of UVB daily for one month. The paraffin sections of skin specimens were stained with hematoxylin-eosin stain for obs... |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |