日本血吸虫成虫在体外培养中产卵和虫卵发育成熟的影响因素研究

在无血清８４１培养基中分别添加胸腺嘧啶核苷（１６×１０－５ｍｏｌ／Ｌ）、鸟氨酸（１０ｍｇ／Ｌ）、强的松龙（１０－５ｍｏｌ／Ｌ）和胰蛋白胨（１０ｇ／Ｌ），可促进日本血吸虫产卵。经１５ｄ的观察证实在８４１培养基中添加上述成份组成的８９１培养基比８４１培养基产卵量高、畸卵率低和产卵高峰持续时间长。成虫最初２４ｈ内产出的虫卵体外培养至第１５ｄ，ＲＰＭＩ１６４０培养基、无血清８４１培养基、８４１培养基和８９１培养基上成熟期虫卵的百分比依次为１１６±４７，１８７±５６，２８４±８３和３０１±７８。

Studies on the Factors which Influenced Oviposition and Maturation of Eggs of Adult Schistosoma Japonicum in Vitro

Separately adding thymidine(1.6×10 -5 mol/L), ornithine(10 mg/L), Prednisoloni(10 -5 mol/L) and tryptone(1.0 g/L) to No.841 culture free of serum can promote oviposition in adult Schistosoma Japonicum. Fifteen days of observation shows that with these ingredients No.891 culture can produce more eggs than No.841 culture, the deformity rate is higher in the former than in the latter and the former ,s oviposition peak lasts longer. The eggs produced by adult Schisfosoma Japonicum within the first 24 hours are developed in vitro for 15 days in RPMI 1640 culture, No. 841 culture free of serum,No. 841 culture and No. 891 culture. The egg percentages in these cultures during the mature period are follows: 11.6±4.7, 18.7±5.6, 28.4±8.3 and 30.1±7.8