A chromatic explosion: the development and future of multiparameter flow cytometry |
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| Authors: | Pratip K. Chattopadhyay Carl‐Magnus Hogerkorp Mario Roederer |
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| Affiliation: | ImmunoTechnology Section, Laboratory of Immunology, Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA |
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| Abstract: | Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a technology that allows us to study the immune system in unprecedented detail. In this article, we review the development of hardware, reagents, and data analysis tools for multiparameter flow cytometry and discuss future advances in the field. Finally, we highlight new applications that use this technology to reveal previously unappreciated aspects of cell biology and immunity. |
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| Keywords: | flow cytometry T cells tetramers fluorochromes data analysis |
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