Rapid peptidomic profiling of peritoneal fluid by MALDI-TOF mass spectrometry for the identification of biomarkers of endometriosis |
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Authors: | Rosa Anna Siciliano Maria Fiorella Mazzeo Valentina Spada Angelo Facchiano Antonio d’Acierno Matteo Stocchero |
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Affiliation: | 1. Institute of Food Sciences, National Research Council (CNR)AvellinoItaly;2. S-IN Soluzioni InformaticheVicenzaItaly |
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Abstract: | Peptidomic profiling of peritoneal fluid by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF-MS) may represent a promising, suitable, rapid method for early diagnosis and staging of endometriosis. In a case-control study, peritoneal fluid was collected from 23 patients affected by endometriosis (eight minimal/mild endometriosis and 15 moderate/severe endometriosis) and six “endometriosis free” women undergoing laparoscopy. MALDI-TOF mass spectra of the peptide fraction extracted from peritoneal fluid samples lead to identify biomarkers potentially suitable for discriminating between peritoneal fluid samples from women affected by minimal/mild endometriosis and those from women affected by moderate/severe endometriosis. Peptidomic analysis of peritoneal fluid samples may define putative peptide biomarkers suitable for staging endometriosis and improve our understanding of the pathogenesis of endometriosis. |
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Keywords: | Biomarkers endometriosis laparoscopy MALDI-TOF-MS peptidomics |
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