冬凌草甲素诱导C6脑胶质瘤细胞凋亡的初步研究

目的:研究冬凌草甲素对大鼠C6脑胶质瘤细胞的抑制增殖及诱导凋亡的作用。方法:不同浓度的冬凌草甲素在不同的时间间隔内作用于C6脑胶质瘤细胞,用冬凌草甲素浓度时间生存曲线来测试冬凌草甲素对C6脑胶质瘤细胞的作用。用流式细胞技术来分析细胞周期的分布情况及凋亡细胞的百分数。结果:生存曲线结果证实冬凌草甲素诱导增殖抑制呈浓度依赖和时间依赖。Hochest 33258斑点染色及流式细胞技术揭示冬凌草甲素诱导C6脑胶质瘤细胞凋亡及抑制其进入细胞周期的G2/M相。结论:冬凌草甲素能够抑制C6脑胶质瘤细胞增殖,诱导细胞凋亡。

Apoptosis of C6 astrocytoma cells induced by oridonin

Objective: To investigate the proliferation suppression and apoptosisinducing effect of oridonin on Rat C6 astrocytoma cells.Methods: C6 cells were treated with different concentrations of oridonin for various time intervals.Oridonin concentration-time viability curve were used to test the effect of oridonin on the C6 cells.The distribution of cell cycle and percentage of apoptosis cells was analyzed with flow cytometry.Results: The results of viability curve demonstrated that oridonin induced suppression of proliferation in a concentration-and time-dependent manner.Hochest 33258 staining and flow cytometry revealed that oridonin induced apoptosis and arrested the entry into G2/M phase of C6 cells.Conclusion: Oridonin can cause the suppression of proliferation and the cell apoptosis in C6 astrocytoma cells.