自发性高血压大鼠NHE-1基因表达与左心室肥厚的相关性

目的：探讨自发性高血压大鼠（SHR）NHE-1基因表达与左心室肥厚的相关性.方法：对SHR和同源血压正常大鼠（WKY）进行平均血压、左室重量（LVW）和体重（BW）的测定，同时采用实时定量荧光检测两组大鼠心肌组织中NHE-1 mRNA的拷贝数.结果：SHR组与WKY组相比，SHR组的LVW、LVW/BW明显高于WKY组（r左室重=0.700，r左室重/体重之比=0.617，P＜0.01），大鼠的平均血压与LVW量、LVW/BW呈正相关（P＜0.01）；SHR组的心肌组织NHE-1 mRNA的表达增高（P＜0.01）.结论：SHR的心肌组织NHE-1 mRNA的表达增高，提示NHE-1可能参与心肌肥厚的发生、发展过程.

Relationship between NHE-1 gene expression in myocardium and left ventricular hypertrophy in spontaneously hypertensive rats

Objective To explore the relationship between NHE-1 gene expression in myocardium and left ventricular hypertrophy in spontaneously hypertensive rats. Methods Body weight (BW), mean blood pressure, and left ventricular weight (LVW) were measured in spontaneously hypertensive rats (SHRs) and normotensive WKY rats. NHE-1 mRNA expression in myocardium was detected by quantitative real-time PCR. Results As compared with those in WKY rats, LVW and LVW/BW ratio in SHRs were obviously increased (r = 0.700, r = 0.617;P < 0.01). Mean blood pressure was positively related with left ventricular weight and the ratio of left ventricular weight to body weight (P < 0.01). The expression of NHE-1 in myocardium was elevated in SHRs (P < 0.01). Conclusion The elevation in NHE-1 mRNA expression in the myocardium of SHRs suggests that NHE-1 gene may be involved in the genesis and development of left ventricular hypertrophy.