Adhesion of Legionella pneumophila on glass and plumbing materials commonly used in domestic water systems |
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| Authors: | Abdelwahid Assaidi Mostafa Ellouali Hassan Latrache Mustapha Mabrouki Mohammed Timinouni Hafida Zahir |
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| Institution: | 1. Laboratory of Bioprocess and Biointerfaces, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, Beni Mellal, Morocco;2. Laboratory of Water Microbiology and Environmental Hygiene, Institut Pasteur du Maroc, Casablanca, Morocco;3. Laboratory of Industrial Engineering, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, Beni Mellal, Morocco;4. Laboratory of Water Microbiology and Environmental Hygiene, Institut Pasteur du Maroc, Casablanca, Morocco |
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| Abstract: | We aimed to investigate the adhesion of Legionella pneumophila serogroup1 and L. pneumophila serogroup2–15 on glass, galvanized steel, stainless steel, copper, Polyvinyl chloride(PVC), Cross-linked polyethylene(PEX-c) and Polypropylene Random Copolymer(PPR). The surface physicochemical properties of both bacterial cells and materials were estimated through contact angle measurements. The roughness and surface topography of the materials were evaluated by Atomic Force Microscopy. The two L. pneumophila serogroups and plumbing materials showed a hydrophobic character, while glass surface was hydrophilic. All strains were adhered to all materials with the exception of copper. The result showed that the adhesion of both L. pneumophila sg1 and sg2–15 was systematically expressed with high intensity on galvanized steel followed by PVC, PEX-c, PPR, stainless steel and the low intensity on glass. The extent of adhesion is in correlation with the surface roughness and acid–bases interactions, while hydrophobicity seems to have no effect in adhesion intensity. |
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| Keywords: | Legionella pneumophila adhesion contact angle roughness AFM |
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