用BrdU标记技术观察大鼠脊髓上胸段灰质细胞的分化发育

目的 观察脊髓灰质细胞的分化发育，寻找脊髓移植时胎鼠脊髓取材的最佳时间。方法 用５－溴－２脱氧尿嘧啶（ＢｒｄＵ）标记ＤＮＡ，ＡＢＣ免疫细胞化学方法观察大鼠上胸段脊髓灰质细胞的增殖分化情况。结果 孕鼠于Ｅ１０ｄｅ腹腔注射ＢｒｄＵ，胎刀脊髓上胸段ＢｒｄＵ免疫反应神经元主要集中在脊髓后角，脊髓侧角的ＢｒｄＵ免疫反应神经元较少，前角仅有少量的ＢｒｄＵ免疫反应神经元；孕鼠于Ｅ１１ｄ腹腔注射ＢｒｄＵ，胎鼠脊髓

APPLICATION OF BrdU LABEL TECHNIQUE FOR STUDYING DIFFERENTIATION AND DEVELOPMENT OF SUPERIOR THORACIC GRAY MATTER OF RAT SPINAL CORD

Objective\ To search for appropriate time of embryonic spinal grafting,we have observed the differentiation and development of cells of spinal gray matter. Methods\ The development and proliferation of spinal gray matter cells of superior thoracic spinal cord were observed by using 5\|bromo\|2\|deoxyuridine(BrdU) to mark DNA and ABC immunocytochemical technique. Results\ When pregnant rat at E10d was given BrdU by intraperitoneal injection,most of BrdU immunoreactive neurons were located in posterior horn of superior thoracic segment of spinal cord in embronic rat,only a few of BrdU immunoreactive neurons appeared in anterior horn and lateral horn.Given BrdU at E11d,the number of BrdU immunoreactive neurons decreased in posterior horn and increased in anterior and lateral horn of superior thoracic segment of spinal cord in embronic rat.Given BrdU at E12d,most of BrdU immunoreactive neurons were mainly found in anterior and lateral horn of superior thoracic segment of spinal cord in embronic rat,only a few of BrdU immunoreactive neurons appeared in posterior horn. Conclusions\ BrdU label technique might be a good method for symbolizing cells of spinal gray matter,the appropriate time of BrdU symbol was at E12d,the perfect time of embryonic spinal graft symbolized by BrdU was at E13d.