结缔组织生长因子在2型糖尿病大鼠虹膜中的表达

目的探索2型糖尿病大鼠虹膜新生血管(new vascularization of iris,NVI)的形成与虹膜组织中结缔组织生长因子(connective tissue growth factor,CTGF)表达的关系。方法将Wistar大鼠40只随机分为实验组和对照组,每组各20只大鼠。实验组大鼠通过腹腔注射STZ制备2型糖尿病大鼠模型,对照组为正常大鼠。造模成功后取出大鼠眼球,将实验组及对照组各取15只眼球用酶联免疫吸附测定法(enzyme linked immunosorbent assay,Elisa)定量分析大鼠虹膜中CTGF的表达情况,实验组及对照组另25只眼球取虹膜进行光镜和电镜观察(其中光镜观察各15只眼球,电镜观察各10只眼球)。结果光镜和电镜下将实验组与对照组相比较,实验组大鼠虹膜有明显的NVI的特征性改变,而对照组无显著改变。在实验组大鼠虹膜中CTGF的含量为(1010.49±8.18)ng·L-1,而在对照组大鼠虹膜中CTGF的含量为(644.82±8.08)ng·L-1,差异有统计学意义(P<0.001)。结论 2型糖尿病大鼠虹膜中CTGF的含量与其NVI的生成有一定关系。

Expression of connective tissue growth factor in iris of rats with type 2 diabetes mellitus

Objective To investigate the correlation between the formation of new vascularization of iris(NVI) and the expression of connective tissue growth factor(CTGF) in iris of type 2 diabetic rats.Methods About 40 Wistar rats were randomly divided into experimental and control groups,20 rats in each group.The experimental group was prepared by intraperitoneal injection of STZ to get the iris neovascularization rat models of type 2 diabetes mellitus.The control group was normal.After the succession of modeling,the eyes were taken out.Fifteen eyes from the experimental group and control group were used enzyme-linked immunosorbent assay for quantitative analysis of the content of CTGF in iris.Another 25 eyes from experimental group and control group were taken the iris for light and electron microscopy(15 eyes for light microscopy and 10 eyes for electron microscopy).Results With comparison between experimental group and control group,light microscopy and electron microscopy showed obvious characteristic changes of NVI in iris of rats in experimental group,while no difference in control group.The contents of CTGF were(1010.49±8.18)ng·L-1 in experimental group and(644.82±8.08)ng·L-1 in control group.There was statistical difference(P<0.001).Conclusion The content of CTGF has relation with formation of NVI in iris of rats with type 2 diabetes mellitus.