中药常山中常山碱和异常山碱的含量测定

 目的 建立高效液相色谱法同时测定中药常山中常山碱和异常山碱总含量的测定方法。方法 采用HPLC测定常山药材和饮片中常山碱和异常山碱的总含量。色谱柱：Kromasil C₁₈(4.6 mm×250 mm，5 μm；流动相：乙腈-水-冰醋酸-三乙胺(9∶91∶0.3∶0.745，冰醋酸调pH值至5.2～6.2之间；流速：1 mL·min-1；检测波长：225 nm；柱温：30 ℃。外标一点法定量。结果 常山碱和异常山碱的混合物在0.021～2.18 μg内呈良好线性关系，方法回收率介于95.16％ ～103.97％之间，回收率的RSD值为3.28％。结论 本法简便、快捷、准确，可用于常山药材及饮片中常山碱和异常山碱的含量测定。

Quantitative Analysis of the Febrifugine and Isofebrifugine Mixture in the Root of Dichroa febrifuga Lour

OBJECTIVE To develop an effective HPLC method for determining the content of febrifugine and isofebrifugine mixture in Dichroa febrifuga root and its Yinpian. METHODS The column used was Kromasil C₁₈(4.6 mm×250 mm，5 μm. The mobile phase consisted of acetonitrile， water， glacial acetic acid and triethylamine(9∶91∶0.3∶0.745， and pH was adjusted with glacial acetic acid to 5.2-6.2. The flow rate was 1 mL·min-1 and the column temperature was 30 ℃. The detection wavelength was at 225 nm. The content was quantified by an external standard method. RESULTS Good linearity of the febrifugine and isofebrifugine mixture was obtained in the range of 0.021-2.18 μg. The method recovery was between 95.16％ and 103.97％， and the RSD was 3.28％. CONCLUSION The method is simple， fast and accurate， and is suitable for the determination of content of febrifugine and isofebrifugine mixture in the Dichroa febrifuga root and its Yinpian.