| 内源性硫化氢在大鼠心力衰竭中的变化及作用 |
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| 引用本文： | 刘国磊,刘青云,陈奎,朱乙声,程智英,张春民,金戈,任永生. 内源性硫化氢在大鼠心力衰竭中的变化及作用[J]. 郧阳医学院学报, 2008, 27(1): 16-18 |
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| 作者姓名： | 刘国磊 刘青云 陈奎 朱乙声 程智英 张春民 金戈 任永生 |
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| 作者单位： | 郧阳医学院临床医学系;郧阳医学院附属东风医院老年科;郧阳医学院生理教研室;2003级;湖北十堰; |
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| 基金项目： | 湖北省十堰市科技攻关项目 , 郧阳医学院基础医学院学生科研项目 |
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| 摘 要： | 目的:观察心肌内源性硫化氢生成的变化及外源性给予硫化氢供体NaHS对心肌损伤的影响,以探讨硫化氢在心力衰竭中的病理生理意义。方法:结扎冠状动脉前降支诱导大鼠心力衰竭,腹腔给予外源性H2S治疗;HE染色检测心肌损伤程度;亚甲基蓝法测定硫化氢浓度;TBA法测定血浆和心肌脂质过氧化产物丙二醛(MDA)含量;自动生化分析仪检测血清天门冬氨酸氨基转换酶(AST)、磷酸肌酸激酶(CPK)及乳酸脱氢酶(LDH)活性。结果:大鼠冠状动脉结扎导致严重心肌损伤;血浆和心肌组织H2S生成显著减少(P<0.001);血浆和心肌MDA含量明显增加(P<0.05)。血浆AST、CPK和LDH活性显著增加(P<0.05)。给予NaHS治疗后,心肌损伤程度明显减轻;血浆和心肌MDA含量明显降低(P<0.05);血浆AST、CPK和LDH活性显著降低(P<0.05)。结论:H2S可通过减少血浆和心肌MDA的生成而对大鼠心肌缺血导致的心力衰竭具有保护作用。
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| 关 键 词： | 硫化氢 心力衰竭 乳酸脱氢酶 丙二醛 |
| 文章编号： | 1006-9674(2008)01-0016-03 |
| 修稿时间： | 2007-12-10 |
Changes and Role of Endogenous Hydrogen Sulfide in Rat with Heart Failure |
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| LIU Guo-lei,LIU Qin-yun,CHEN Kui,ZHU Yi-sheng,CHEN Zhi-ying,ZHANG Chun-ming,JING Ge,REN Yong-sheng. Changes and Role of Endogenous Hydrogen Sulfide in Rat with Heart Failure[J]. Journal of Yunyang Medical College, 2008, 27(1): 16-18 |
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| Authors: | LIU Guo-lei LIU Qin-yun CHEN Kui ZHU Yi-sheng CHEN Zhi-ying ZHANG Chun-ming JING Ge REN Yong-sheng |
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| Affiliation: | 1Class 1 grade 2003;Department of Clinical Medicine;Dongfeng Hospital;2Department of Gerontology;3Department of Physiology;Yunyang Medical College;Shiyan;Hubei 442000;China |
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| Abstract: | Objective To investigate the changes of endogenous hydrogen sulfide(H2S)in rat with heart failure(HF)and the effects of exogenous administration of H2S donor sodium hydrosulphide(NaHS)on myocardial infarction,so as to explore the pathophysiological significance of H2S in HF.Methods: The experimental HF model was induced by left anterior descending coronary occlusion and then was treated with intraperitoneal injection of H2S;the degree of myocardial infarction was determined by observing hematoxylin-eosin st... |
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| Keywords: | Hydrogen sulfide Heart failure Malondialdehyde Lactate dehydrogenase |
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