| DNA损伤诱导细胞周期解偶联 |
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| 引用本文： | 鞠桂芝,付士波,闫凤琴,李鹏武.DNA损伤诱导细胞周期解偶联[J].吉林大学学报(医学版),2004,30(5):665-667. |
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| 作者姓名： | 鞠桂芝 付士波 闫凤琴 李鹏武 |
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| 作者单位： | 吉林大学公共卫生学院 卫生部放射生物学重点实验室, 吉林 长春130021 |
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| 摘 要： | 目的：观察DNA损伤因子丝裂霉素 (MMC) 能否诱导细胞周期解偶联。
方法：采用PI荧光标记及流式细胞术 (FCM) 测定细胞周期并分析细胞倍体的变化。
结果：1.0及2.0 mg?L-1 MMC作用后24 h, SKOV-3细胞八倍体数显著增高(P<0.01, P<0.05)。1.0、2.0及4.0 mg?L-1 MMC作用后24 h，L929及EL-4细胞八倍体数显著增高(P<0.05，P<0.01，P<0.01及P<0.01，P<0.05，P<0.01)。结论：MMC可诱导人卵巢癌细胞SKOV-3、小鼠成纤维细胞L929及小鼠淋巴瘤细胞EL-4发生细胞周期解偶联。
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| 关 键 词： | 丝裂霉素 细胞周期 解偶联 |
| 文章编号： | 1671-587X(2004)05-0665-03 |
| 收稿时间： | 2004-03-21 |
| 修稿时间： | 2004年3月31日 |
Cell cycle uncoupling indued by DNA damage |
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| JU Gui-zhi,FU Shi-bo,YAN Feng-qin,LI Peng-wu.Cell cycle uncoupling indued by DNA damage[J].Journal of Jilin University: Med Ed,2004,30(5):665-667. |
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| Authors: | JU Gui-zhi FU Shi-bo YAN Feng-qin LI Peng-wu |
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| Institution: | MH Radiobiology Research Unit, School of Public Health,Jilin University, Changchun 130021, China |
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| Abstract: | Objective To investigate whether cell uncoupling could be induced by mitomycin C (MMC). Methods Cell cycle was analyzed by flow cytometry (FCM) following staining of cells with propidium iodide (PI) and the changes of polyploid cells were observed.Results The 8N cells were increased significantly compared with control,24 hours after exposure of SKOV-3 cells to MMC with the doses of 1.0 and 2.0 mg·L -1 (P<0.01, P<0.05, respectively). And 24 hours after exposure of L929 cells and EL-4 cells to MMC with the doses of 1.0, 2.0, and 4.0 mg·L -1 , the 8N cells were also increased significantly compared with control (P<0.05 or P<0.01). Conclusion The cell cycle uncoupling could be induced by MMC exposure. |
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| Keywords: | DNA damage mitomycin C cell cycle uncoupling |
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