CLINICAL APPLICATION OF FLOW CYTOMETRY IN LIVE RELATED DONOR RENAL TRANSPLANTATION |
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| Authors: | GS CHOPRA AS NARULA SC DASH NK MEHRA |
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| Affiliation: | *Classified Specialist Pathology, Army Hospital, Delhi Cantt;+Classified Specialist Medicine & Nephrology, Army Hospital, Delhi Cantt;#Profcssor and Head, Department of Nephrology, All India Institute of Medical Sciences, New Delhi;**Professor and Head, Department of Histocompatibility & Immunogeneties, All India Institute of Medical Sciences, New Delhi |
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| Abstract: | To monitor the activity of the humoral component in graft dysfunction following renal transplantation using live related donors, flowcytometric cross-match procedure was adopted. Antidonor antibodies were detected in the sera of both pre- and post-transplant patients using conventional serological cytotoxicity cross-match and flowcytometric cross-match assays. In the 52 pretransplant samples no significant differences were observed in flowcytometric and cytotoxicity tests except in 2 secondary transplant cases which were negative by cytotoxicity test. However, in post-transplant samples, floweytometry was found to be a more objective and useful test than cytotoxicity testing in distinguishing 6 out of 7 mild acute-graft-rejection episodes. Both tests were found to be negative in alt 5 cases of cyclosporin-A nephrotoxicity, 7 cases of acute tubular necrosis and 10 out of 11 cases of chronic rejection.KEY WORDS: Flowcytometry, Cross-match, Graft dysfunction, Kidney transplantation |
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