巨噬细胞与动脉粥样硬化斑块稳定性

为探讨抗氧化剂维生素Ｅ和元素硒对脂质过氧化诱导内此细胞表达细胞粘附分子及单核细胞粘附的影响产胺诱发培养内皮细胞脂质过氧化，检测维生素Ｅ和元素硒对单核细胞会的影响，用免疫组化及共焦显微镜观察维生素Ｅ和元素硒对内此表达血管细胞粘附分子－１及内此细胞白细胞粘附分子－１的影响。结果显示抗氧化剂维生素Ｅ和元素硒可抑制内此脂质过氧化，抑制血管细胞粘附分子－１及内此占附分子－１表达，减少单核细胞粘附。提示抗氧化

Effect of Antioxidant on the Expression of Cellular Adhesion Molecules in Cultured Endothelial Cells Induced by Peroxidation

Aim To investigate the effect of antioxidant Vitamine E and Selenium on the expression of cellular adhesion molecules(CAMs) induced by lipid peroxidation(LPx) in cultured endothelial cells and monocytes(MC) adherence to EC. Methods LPx was initiated withdiamide(DM).Effect of Vitamine E and Selenium on the expression of vascular adhesion molecule-1(VCAM-1) and endothelial leucocyte adhesion molecule-1(ELAM-1) was studied under laser confocal microscope,MC adherence to EC was measured. Results Vitamine E and Selenium could decrese the content of lipid peroxid,reduce VCAM-1 and ELAM-1 expression andMC adherence to EC incubated with DM. Conclusion Antioxidant could inhibite VCAM-1 and ELAM-1expression induced by LPx to reduce Mc adherence to EC and may possiboly prevent the development of athrosclerosis (As) lesion.