慢性牙周炎患者治疗前后患牙中IL-6、IL-22及IL-4表达水平的研究

目的:探讨白细胞介素-6(IL-6)、白细胞介素-22(IL-22)、白细胞介素-4(IL-4)与慢性牙周炎的关系。方法:选取我院进行治疗的48例慢性牙周炎患者为治疗组,全部常规治疗,正常健康对照组32例,记录正常对照组及治疗组治疗前、后的临床指数,采用ELISA检测治疗组治疗前、后及正常对照组龈沟液(GCF)及血清中IL-6、IL-22及IL-4的含量,并讨论与临床指数的相关性。结果:IL-6、IL-22及IL-4含量与正常对照组比较,治疗组治疗前及治疗后6周差异有统计学意义(P均<0.01),治疗后12周接近正常对照组值,差异无统计学意义(P均>0.05);治疗后两组与治疗前比较,各项指标差异均有统计学意义(P均<0.01)。IL-6、IL-22及IL-4含量与牙周探诊深度(PD)治疗前、后比较均有线性显著相关(P均<0.05),与菌斑指数(PⅡ)治疗前、后比较均无线性显著相关(P均>0.05);IL-6与IL-22、IL-4与IL-22分别比较,均有线性显著负相关(P均<0.05),IL-6与IL-4含量在治疗前、后比较有线性显著正相关(P均<0.05)。结论:IL在牙周炎的免疫保护或免疫病理过程中起着重要作用。

Expression level of IL-6,IL-22 and IL-4 in chronic periodontitis teeth before and after treatment

Objective: To investigate the expression level of IL-6,IL-22 and IL-4 chronic periodontitis teeth before and after treatment.Method: Forty-eight patients with chronic periodontitis that were treated in our hospital were selected as treatment group,which were given conventional treatment,and 32 healthy volunteers were enrolled as control group.Concentrations of IL-6,IL-22 and IL-4 in serum and GCF as well as other clinical indexes before and after treatment were detected with ELISA Results:Significant difference in IL-6,IL-22 and IL-4 concentrations were found between the two groups,and the concentrations of the treatment group before treatment was also significant different from that 6 weeks after treatment(P all <0.01).After 12 weeks of treatment,IL-6 and IL-22,IL-4 and IL-22 levels were similar in treatment group and control group(P all >0.05).Significant linear correlation was found between IL-6 and IL-22,IL-4 and IL-22 levels and PD before and after treatment,but no similar correlation was found between those levels and PⅡ(P>0.05).Positive linear correlation was found among IL-6 and IL-22,IL-4 and IL-22 levels(P<0.05),while negative correlation was found between IL-6 and IL-4 before and after treatment(P<0.05).Conclusions: IL plays an important role in protective immunity or immune pathological process in periodontitis.