支原体肺炎患儿TNF-α、IL-6及IL-10的变化及其临床意义

目的:探讨由支原体引起的肺炎对患儿肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)水平的影响及其在临产诊断中的应用。方法:选择2009年3月～2011年5日,河北省儿童医院收治的76例支原体肺炎患儿作为观察组,同时选择同时期在我院体检的健康儿童76例作为健康对照组,采用双抗体夹心ELISΑ法检测两组儿童血清和支气管肺泡灌洗液(BALF)中TNF-α、IL-6和IL-10的水平,利用Pearson Correlation对TNF-α、IL-6和IL-10与儿童支原体肺炎的相关性进行分析。结果:观察几组血清中TNF-α、IL-6和IL-10水平分别为(19.24±7.74)、(44.91±18.76)和(15.78±6.54)pg/mL,显著高于健康儿童组(P<0.05);观察组BALF中TNF-α、IL-6和IL-10水平分别为(31.16±10.54)、(78.44±22.58)、(21.67±9.80)pg/mL,显著高于健康儿童组(P<0.05);血清和BALF中TNF-α、IL-6和IL-10的相关系数均大于0.9,均达到显著水平(P<0.05)。结论:支原体肺炎患儿血清和支气管肺泡灌洗液中TNF-α、IL-6及IL-10的水平与支原体肺炎存在显著相关性,其水平可为支原体肺炎诊断和治疗提供理论依据和参考。

Changes in serum TNF-a,IL-6 and IL-10 levels and its significance in children with mycoplasma pneumonia

Objective: To discuss the changes of serum TNF-a,IL-6 and IL-10 levels in children with Mycoplasma pneumoniae pneumonia and its significance.Methods:Seventy-six cases of children with Mycoplasma pneumoniae pneumonia that were treated in Hebei Children's Hospital during March 2009 and May 2011 were selected as observation group,and 76 health children were also selected as control group.The double antibody sandwich ELIS method was used to detect the TNF-α,IL-6 and IL-10 levels in serum and bronchoalveolar lavage fluid(BALF) in both groups.The Pearson Correlation was used to analyze the correlation between TNF-α,IL-6 and IL-10 and the occurrence of Mycoplasma pneumoniae pneumonia.Results:Serum TNF-α,IL-6 and IL-10 levels of the observation group was(19.24± 7.74) pg/mL,(44.91 ±18.76) pg/mL and(15.78± 6.54) pg/mL,significantly higher than that of healthy children group(P<0.05).The TNF-α,IL-6 and IL-10 level of BALF in the observation group children was(31.16± 10.54) pg/mL,(78.44 ±22.58) pg/mL and(21.67± 9.80) pg/mL,significantly higher than that of healthy children group(P<0.05).The correlation coefficients between TNF-α,IL-6 and IL-10 in serum and BALF were all greater than 0.9 which reached the significant level(P<0.05).Conclusions:There were significant correlation between TNF-α,IL-6 and IL-10 in serum and BALF.The level TNF-α,IL-6 and IL-10 level of serum and BALF could be used as reference for the diagnosis and treatment of Mycoplasma pneumoniae pneumonia.