AKT2 S100A4 及MMP-9 在鼻咽癌组织中的表达*

目的:探讨AKT 2、S100A4 及MMP-9 在鼻咽癌组织中的表达、相互关系及临床意义。方法:应用免疫组化EliVision 二步法检测50例鼻咽癌组织、25例慢性鼻咽炎组织标本中AKT 2、S100A4 及MMP-9 蛋白的表达，并分析三者与鼻咽癌患者临床特征的关系及三者表达的相关性。结果:鼻咽癌组织中AKT 2、S100A4 及MMP-9 的阳性表达率分别为48.0%（24/50）、52.0%（26/50）、74.0%（37/50），显著高于慢性鼻咽炎组织的8.0%（2/25）、0（0/25）、36.0%（9/25），P 均<0.01；AKT 2 和S100A4 的表达均与鼻咽癌颈淋巴结转移及临床分期相关（P 均<0.05），MMP-9 的表达与鼻咽癌颈淋巴结转移相关（P<0.05），而三者的表达与患者性别、年龄及T 分期无关（P 均>0.05）；鼻咽癌组织中AKT 2 和S100A4 与MMP-9 的表达呈正相关（P 均<0.01），AKT 2 与S100A4 的表达无关（P>0.05）。 结论:AKT 2、S100A4 及MMP-9 的高表达在鼻咽癌的发生、发展及转移方面可具有重要作用，AKT 2 和S100A4 可能通过上调MMP-9 的表达而促进鼻咽癌的发展及转移，联合检测三者的表达情况对判断鼻咽癌生物学行为和预测肿瘤转移有一定的临床价值。 

Expression of AKT2,S100A4 and MMP-9 in Nasopharyngeal Carcinoma

Objective:To investigate the expression of AKT2, S100 A4 and MMP- 9 in nasopharyngeal carcinoma (NPC) and its clinical significance. Methods:Immunohistochemical technology (EliVision two steps) was applied to detect the ex -pression of AKT2, S100 A4 and MMP- 9 proteins in 50NPC tissue samples and25tissue samples of chronic nasopharyngi -tis. The relationship of AKT2, S100 A4 and MMP- 9 expression with clinical features of NPC and the correlations among the three proteins were analyzed. Results: The positive rates of AKT 2 (48.0% ,24/50), S 100 A4 (52.0% ,26/50) and MMP- 9 (74.0%,37/50) in NPC tissues were respectively higher than those in chronic nasopharyngitis tissues ( 8.0%,2/25), (0%,0/25), and (36.0%,9/25), P <0.01]. The positive rates of AKT 2 and S 100 A4 were correlated with cervical lymph node metasta -ses and clinical stage (P<0.05) The positive rate of MMP- 9 was correlated with cervical lymph node metastases (P<0.05). The positive expression of the three proteins was not correlated with gender, age or T classification (P>0.05). The expres -sion of AKT2 and S 100 A4 was positively correlated with MMP-9 expression in NPC tissues (P<0.01). But the expression of AKT 2 was not correlated with S100 A4 expression (P>0.05). Conclusion:The increased expression of AKT2, S 100 A4 and MMP-9 may play a significant role in the occurrence, development and metastasis of NPC. AKT2 and S 100 A4 may pro-mote the development and metastasis of NPC by up-regulating the expression of MMP-9. Detection of AKT2, S100 A4 and MMP-9 is of great value for predicting the biological behaviors and metastasis of NPC.