重组生长激素促短肠大鼠结肠代偿机制的探讨

目的　探讨重组生长激素促短肠大鼠结肠代偿的作用机制。方法　将短肠大鼠分成肠内营养（EN）和肠内营养+重组生长激素（EG）两组。结果　自术后第15天起，EG组体重的减轻明显低于EN组(P＜0.05)，术后第21天起体重已大于术前；EG组氮平衡的改善明显好于EN组；血浆蛋白水平也高于EN组(P＜0.05)；EG组结肠壁胰岛素样生长因子1(IGF-1)mRNA含量比EN组明显升高〔(1291±43）vs.（1026±42），P＜0.05〕，IGF-1受体mRNA含量明显降低〔(899±5）vs.（1113±7），P＜0.05〕;EG组的血IGF-1水平明显高于EN组〔(455±107）ng/mlvs．（329±68）ng/ml，P＜0.05〕，GH水平也显著升高〔(9.7±3.3）ng/mlvs.（5.8±2.4）ng/ml，P＜0.05〕。结论　重组生长激素可促使短肠大鼠IGF-1和结肠壁IGF-1mRNA的合成及残留肠道对营养物质吸收。

The mechanism of rGH in the promotion of colonic adaptation in short bowel rats

Objective To study the mechanism of rGH in the pr omotion ofcolonic morphologic adaption in short bowel rats． Method Twenty rats undergoing 80～85% small bowel res ection were randomized into two groups receiving enteral nutrition (EN), and en teral nutrition plus GH (EG). Ten normal rats served as control (CONT). Result On post-op day 15, body weight in EG group was heavier than in EN group (P＜0.05). The net nitrogen gain in EN group was less than that in CONT (P<0.05) and in EG group (P＜0. 01).The serum protein level of EG group was higher than EN group(P＜0.05) . The level of colonic muscoal IGF-1 mRNA was higher in EG than in EN (1291 ±43 vs. 1026±42，P＜0.05), whereas the level of colonic muscoal IGF-1 receptor mRNA was lower than normal (899±5 vs. 1113±7，P＜0.05) . The levels of serum insulin-like growth factor-1 (IGF-1) of EG group was hi gher than EN(455±107ng/ml vs． 329±68ng/ml，P＜0.05). The leve l of serum GH of EG group was higher than EN(9.7±3.3ng/ml vs． 5.8±2.4 ng/ ml，P＜0.05). Conclusions rGH promotes the synthesise of serum IGF-1 and colonic muscoal IGF-1 mR NA, henc e significantly elevates the colonic functional adaption to absorb nutrients.