| 建立大鼠原位肠-肝灌流模型评价绿原酸的代谢 |
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| 引用本文： | 王素军,张志伟,赵艳红,张振清,谢海棠,阮金秀. 建立大鼠原位肠-肝灌流模型评价绿原酸的代谢[J]. 中国临床药理学与治疗学, 2006, 11(12): 1340-1344 |
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| 作者姓名： | 王素军 张志伟 赵艳红 张振清 谢海棠 阮金秀 |
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| 作者单位： | 1. 军事医学科学院毒物药物研究所药物代谢动力学重点实验室,北京,100850
2. 安徽省药物临床评价中心,皖南医学院附属弋矶山医院,芜湖,241001,安徽 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的：建立大鼠原位肠-肝灌流模型，运用该模型研究绿原酸在大鼠肠、肝中的代谢转化。方法：采用液相色谱质谱联用（HPLC-MS）法测定大鼠原位肠-肝灌流模型灌流液中绿原酸及其代谢产物咖啡酸、阿魏酸和马尿酸。结果：绿原酸十二指肠给药后，灌流液中主要活性代谢产物为阿魏酸，同时在灌流液中也检测到少量活性代谢产物咖啡酸和大量代谢终产物马尿酸。结论：大鼠原位肠-肝灌流模型适用于绿原酸生物转化和代谢动力学的研究；绿原酸在肠中存在广泛的代谢。
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| 关 键 词： | 大鼠原位肠-肝灌流模型 绿原酸 咖啡酸 阿魏酸 马尿酸 代谢 |
| 文章编号： | 1009-2501(2006)12-1340-05 |
| 收稿时间： | 2006-10-10 |
| 修稿时间： | 2006-11-30 |
Establishment of in situ perfused rat intestine-liver model for evaluation on chlorogenic acid metabolism |
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| WANG Su-jun,ZHANG Zhi-wei,ZHAO Yan-hong,ZHANG Zhen-qing,XIE Hai-tang,RUAN Jin-xiu. Establishment of in situ perfused rat intestine-liver model for evaluation on chlorogenic acid metabolism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(12): 1340-1344 |
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| Authors: | WANG Su-jun ZHANG Zhi-wei ZHAO Yan-hong ZHANG Zhen-qing XIE Hai-tang RUAN Jin-xiu |
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| Affiliation: | 1. Key Lab of Drug Metabolism and Pharmacokinetics , Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China ; 2.Anhui Provincial Center for Drug Clinical Evaluation, Affiliated Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China |
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| Abstract: | AIM: To establish a model of in situ perfused rat intestine-liver preparation and HPLC-MS method in the determination of chlorogenic acid, caffeic acid, hippuric acid and ferulic acid in perfusate, and investigate chlorogenic acid metabolism in intestine and liver of rats by the model. METHODS: Chlorogenic acid and its metabolites (caffeic acid, ferulic acid and hippuric acid) were detected by HPLC-MS from samples of in situ perfused rat intestine-liver model. RESULTS: Main active metabolite of chlorogenic acid is ferulic acid in in situ perfused rat intestine-liver model after intraduodenal injection to rats, and trace caffeic acid and abundant hippuric acid were also found in perfusate. CONCLUSION: In situ perfused rat intestine-liver models are suitable for the study of metabolism of chlorogenic acid in intestine and liver of rats. Chlorogenic acid was extensively metabolized in rat intestine. |
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| Keywords: | in situ perfused rat intestine-liver model chlorogenic acid caffeic acid ferulic acid hippuric acid metabolism |
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