TT病毒粪-口传播途径的初步探讨

①目的 探讨TT病毒（TTV）经粪－口传播的可能性。②方法 采用半巢式聚合酶链反应，检测6例TTV感染的肝病病人粪便标本中TTV－DNA，测序，与N22和TA278株核苷酸比较并进行基因分型，探讨粪便中TTV－DNA检出率与血清中病毒滴度的关系。③结果 血清病毒滴度较高的2例病人（10^7/L,10^6/L)的粪便标本中检出了TTV－DNA，滴度分别为10^7/L,10^6/L，基因型分别为G1a,G1b；在血清病毒滴度为10^4/L的4例病人中，仅1例在粪便中检出了TTV－DNA，滴度为10^4/L，基因型为G2。④结论 肝病病人粪便标本中TTV含量与血清滴度直接相关，TTV具有经粪－口传播的可能性。

A PRELIMINARY STUDY ON FECAL-ORAL TRANSMISSION OF TT VIRUS

Objective To study the possibility of feca l oral transmission of TT virus(TTV). Methods Paired feces and serum samples from 6 patients with type B and/or type C hepatitis were tested for TTV DNA and their titers by PCR with s eminested primers. Genotypes were determined by their sequences compared with th e original N22 and TA278 strains. ResultsTTV DNA was detected in fecal specimen of two patients wh o had high viral titers in serum(10 7/L,10 6/L); the genotypes were G 1a and G 1b , respectively. In 4 patients with viral titer of 10 4/L, TTV DNA was detected in only one fecal specimen. The genotype was G 2. Conclusion The amount of TTV in fecal specimens of patients with hepatitis is directly correlated to serum titer, indicating that TTV can be tran smitted by fecal oral route. [