肋软骨膜在肋骨软骨移植重建下颌髁突过程中对髁突改建的影响

目的 对比观察带肋软骨膜的肋软骨移植重建下颌髁状突过程中软骨组织学变化及骨形态发生蛋白质-2(BMP-2)的表达情况,探讨肋软骨膜在肋软骨移植改建髁状突过程中作用的机制及意义。方法 以健康新西兰白兔为研究对象,制作带肋软骨膜(实验组)和不带肋软骨膜(对照组)的肋骨软骨移植重建髁状突的动物模型,采用组织学观察和免疫组织化学的方法,检测重建后不同阶段(2、4、8周)软骨组织学变化及其BMP-2的表达。结果 组织学观察显示实验组软骨组织内细胞增殖明显早于对照组。重建的髁状突软骨组织内BMP-2表达程度存在差异:在2、4、8周时实验组BMP-2的表达明显高于对照组,差异有统计学意义。结论 肋软骨膜在肋软骨移植重建下颌髁状突过程中可能对软骨生长和改建有促进作用。

Effect of costal perichondrium on reconstruction of mandibular condyle with Costochondral grafts

Objective To explore the mechanism about costal perichondrium regulating reconstruction of Costal Cartilage by observing the histological expressions and expressions of BMP-2 of the Costal Cartilage.Methods Rabbits were devided randomly into the experiment group and the control group.For the experiment group,costal perichondrium was protected in the operation of Costochondral reconstruction of mandibular condyles, while for the control group, costal perichondrium was removed.Histological observation and immunohistochemics were used to detect the histological changes and expressions of BMP-2 during different stages.Results Histological observation showed that the proliferation of the chondrocytes in the experiment group happened earlyer than that in the control group.The expressions of BMP-2 of the Costal Cartilage showed that there was more positive results in the experiment group,with statistical significance.Conclusion Costal perichondrium has a beneficial function in regulating proliferation and reconstructing Cartilage.