CpG ODN对哮喘气道MMP-9表达的影响

目的：探讨非甲基化胞嘧啶-鸟嘌呤的寡核苷酸链（CpG ODN）对哮喘气道重塑和气道基质金属蛋白酶-9（MMP-9）表达的影响。方法：以卵蛋白致敏激发的小鼠慢性哮喘模型为对象，观察对照组、哮喘组、CpG ODN组及地塞米松组之间支气管壁厚度改变及气道MMP-9表达。结果：（1）CpG ODN组和地塞米松组的支气管壁厚度大于对照组而小于哮喘组（P〈0．05），而CpG ODN组和地塞米松组间则无显著差异（P〉0．05）；（2）CpG ODN组和地塞米松组MMP-9表达高于对照组（P〈0．05）而小于哮喘组（P〈0．05），CpG ODN组和地塞米松组间则无显著差异（P〉0．05）。结论：慢性哮喘小鼠气道壁明显增厚，而早期行CpG ODN干预则可抑制肺内MMP-9的表达而减轻气道重塑。

Effect of CpG ODN on the expression of MMP-9 in murine model of chronic asthma

Objective To study the effects of CpG oligodeoxynuleotides(ODN) on airway remodeling and expression of matrix metalloprotease-9(MMP9) in a murine model of chronic asthma.Methods BALB/c mice were sensitized and repeatedly challenged with ovalbumin.Pathological slides prepared were stained with hematoxylin-eosin.The thickness of airway wall was measured.The expressions of MMP-9 were detected by immunohistochemistry.Results(1)The thickness of airway wall of CpG ODN and dexamethasone(DXM) treatment groups was higher than control group(P<(0.05)),while lower than asthma group(P<0.05).But it had not statistically significant difference between CpG ODN group and DXM treatment group(P>0.05).(2)The expressions of MMP-9 in the CpG ODN and DXM treatment groups were higher than in control group(P<0.05),while lower than in asthma group(P<0.05).It had not significant difference between CpG ODN group and DXM treatment group(P>0.05).Conclusion Airway remodeling can occur in the chronic asthma mice.Early intervention with steroid or CpG ODN may partially inhibit the expressions of MMP-9 in the lungs of chronic asthma mice,and airway remodelings are abated.