| 人大网膜间充质干细胞的体外培养、鉴定及诱导化分方法 |
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| 引用本文： | 王滔,邹浩,张小文,纳钊,沈需,魏东. 人大网膜间充质干细胞的体外培养、鉴定及诱导化分方法[J]. 昆明医学院学报, 2011, 32(2): 11-15 |
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| 作者姓名： | 王滔 邹浩 张小文 纳钊 沈需 魏东 |
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| 作者单位： | 1. 昆明医学院,云南昆明,650031
2. 昆明医学院第二附属医院肝胆外二科,云南昆明,650101 |
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| 基金项目： | 云南省科技厅-昆明医学院联合专项基金项目资助 |
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| 摘 要： | 目的建立人大网膜脂肪问充质干细胞（adiposederivedIIlesechymalSteITIcells，ADSCs）的原代培养及诱导分化为脂肪细胞的方法．方法术中取人大网膜脂肪组织，采用胶原酶消化法原代培养，经流式细胞仪鉴定细胞CD44、CD34、CD90和CD45分子、MTTr法测定细胞生长曲线，取P3细胞以3-异丁基-1-甲基黄嘌呤、胰岛素、地塞米松及吲哚美辛诱导该细胞向脂肪细胞分化，油红O脂肪染色法进行脂肪细胞鉴定．结果培养卅的细胞形态均-呈梭形或漩涡状生长，经流式细胞仪鉴定为ADSCs，细胞生长曲线表明P3细胞增值旺盛，用分化培养基诱导后，细胞形态由梭形变为圆形，细胞体积增大，胞浆内聚集脂肪颗粒，油红0染色鉴定为成熟的脂肪细胞．结论该方法能培养出形态均一的人大网膜ADSCs，经诱导后可向成熟脂肪细胞分化．
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| 关 键 词： | 脂肪问充质干细胞 诱导分化 鉴定 |
Cultivation,Differentiation and Identification of Human Omental Adipose Derived Mesenchymal Stem Cells in Vitro |
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| WANG Tao,ZOU Hao,ZHANG Xiao-wen,NA Zhao,SHEN Xu,WEI Dong. Cultivation,Differentiation and Identification of Human Omental Adipose Derived Mesenchymal Stem Cells in Vitro[J]. Journal of Kunming Medical College, 2011, 32(2): 11-15 |
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| Authors: | WANG Tao ZOU Hao ZHANG Xiao-wen NA Zhao SHEN Xu WEI Dong |
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| Affiliation: | WANG Tao1),ZOU Hao2),ZHANG Xiao-wen2),NA Zhao1),SHEN Xu1),WEI Dong2)(1)Kunming Medical University,Kunming Yunnan 650031,2)The 2nd Dept.of Hepatobiliary Surgery,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China) |
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| Abstract: | Objective To establish the primary culture and induced differentiation to fat cells of human omental adipose derived mesechymal stem cells(ADSCs).Methods The omental adipose tissues were obtained by opration and fibroblast-like cells was primarily cultured by collagenase digestion.The ADSCs were identificated by detecting the expression of CD44,CD34,CD90 and CD45 with flow cytometry,cell growth curve was measured by MTT method,the P3 cells were induced to fat cells with 3-isobutyl-1-methylxanthine,insulin,d... |
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| Keywords: | ADSCs Induced differentiation Identification |
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