Kir2.1和Kir3.1钾通道mRNA在大鼠平滑肌组织中的比较表达

目的　研究Kir2 1和Kir3 1mRNA在平滑肌及其它组织中表达的差异。方法　用RT PCR方法检测mRNA的表达 ,用光密度扫描法半定量测定表达差异。结果　在大鼠的左心室、大脑皮层、主动脉平滑肌、膀胱平滑肌和呼吸道平滑肌上均有Kir2 1和Kir3 1的表达 ,通过光密度扫描分析显示Kir2 1mRNA在各组织的表达水平 (Kir2 1/GAPDH)依次为主动脉平滑肌 >心脏 =大脑皮层 =气管平滑肌 >膀胱平滑肌 ;Kir3 1mRNA的表达水平 (Kir3 1/GAPDH)依次为主动脉平滑肌 =气管平滑肌 >心脏 =大脑皮层 =膀胱平滑肌。结论　Kir2 1和Kir3 1在不同组织中2 0 0 0 0 4 0 5收稿 国家自然科学基金资助项目 ,No 39770 85 9;国家重点基础研究“973”子课题 ,NoG19980 5 110 6作者简介 :任亚军 ,男 ,30岁 ,博士研究生 ;王晓良 ,男 ,45岁 ,PhD (德国 ) ,研究员 ,博士生导师 ,中国药理学会常务理事的表达量是有差异的 ,这种差异有内在的生理意义。

Comparative mRNA expressions of Kir2.1 and Kir3.1 potassium channels in rat smooth muscle tissues

AIM To study mRNA expression difference of Kir2 1 and Kir3 1 potassium channels in rat smooth muscle and other tissues. METHODS mRNA expressions were detected by RT PCR, comparative expression levels were determined by imaging densitometer. RESULTS Both Kir2 1 and Kir3 1 mRNA were expressed in rat left ventricle, cerebral cortex, aortal smooth muscle, urinary bladder smooth muscle and airway smooth muscle. The relative expression levels of Kir2 1(Kir2 1/GAPDH) were aortal smooth muscle>left ventricle=cerebral cortex=airway smooth muscle>urinary bladder smooth muscle; the relative expression levels of Kir3 1 (Kir3 1/GAPDH)were aortal smooth muscle= airway smooth muscle>left ventricle=cerebral cortex =urinary bladder smooth muscle. CONCLUSION mRNA expression levels of Kir2 1 and Kir3 1 in different tissues were different, this different expressions have physiological significance.