白细胞介素-1对骨髓基质细胞膜电位的影响及其与Cl~-通道的关系

目的　研究重组人白细胞介素 1β(IL 1β)对骨髓基质细胞 (BMSC)膜电位的影响及其与Cl-通道的关系 ,探讨离子通道是否参与细胞因子IL 1的生物信号转导。方法　用荧光染料DiBAC4 (3)标记原代培养的BMSC ,在激光扫描共聚焦显微镜下直接监测IL 1β刺激下细胞膜电位的实时动态变化以及Cl-通道阻断剂呋喃苯胺酸 (furosemide)对IL 1β膜电位效应的影响。结果　IL 1β加入测定体系后浓度依赖性地引起BMSC膜电位的迅速改变。低浓度时 (8～ 10 0kU·L-1)发生去极化反应 ,高浓度时 (2 0 0～ 5 0 0kU·L-1)发生超极化反应。非受体方式作用的IL 1β肽段 16 3～ 171对膜电位无影响。Furosemide(2 0～ 5 0 0 μmol·L-1)浓度依赖性地抑制IL 1β的膜电位效应。结论　膜电位的变化为IL 1受体后早期信号事件 ,Cl-通道的活化参与了IL 1的生物信号转导。

Effect of interleukin-1 on membrane potential of bone marrow stromal cell and its correlation with Cl - channel

AIM To study the effect of recombinant human interleukin 1β (IL 1β) on membrane potential of mice bone marrow stromal cells ( BMSC ) and related ion events. METHODS DiBAC 4(3) loaded BMSC was measured by laser scanning confocal microscope and Cl - channel blocker furosemide was used in some experiments. RESULTS IL 1β induced a rapid change of membrane potential, with depolarization at low concentration (8～100 kU·L -1 ) and hyperpolarization at high concentration (200～500 kU·L -1 ). IL 1β fragment 163～171 had no effect on membrane potential of BMSC. Cl - channel blocker furosemide(20～500 μmol·L -1 ) dose dependently inhibited the effect of IL 1β. CONCLUSION Change of membrane potential is the early signal of IL 1 and Cl - channel is concerned in the ion mechanism.