血管内皮生长因子基因多态性与重症早产儿视网膜病相关性的meta分析

目的评价血管内皮生长因子（VEGF）基因多态性与重症早产儿视网膜病（ROP）的相关性。方法以“血管内皮生长因子、早产儿视网膜病和多态性”（中英文）为主题词联合检索Cochrane图书馆、Medline、PubMed、EMBASE、中国期刊全文数据库、万方数据库及中国生物医学文献数据库，检索起止时间均为建库至2013年12月，并且对重要文献的参考文献采取手工回溯检索。获取VEGF与重症ROP相关性的病例对照研究。对文献进行质量评价。采用State10．0软件进行异质性检验，根据检验结果选择适当的效应模型进行meta分析。结果共9篇文献（9项独立研究）纳入分析，样本量l745例，漏斗图检验不存在发表偏倚。Meta分析结果显示：VEGF基因中的3个等位基因变异：405G→C（rs2010963）、-460T→C（rs833061）和936c→T（rs3025039）与重症ROP患者无相关性，OR值分别为0．95（95％（21：0．67-1．36，P=0．79）、1．20（95％CI：0．89～1．61，P=0．23）和1．23（95％CI：0．43～3．53，P=0．7）。结论VEGF基因405G→C（rs2010963）、-460T→C（rs833061）和936C→T（rs3025039）多态性与重症ROP无相关性。

Meta analysis of relationship between polymorphism of genes of vascular endothelial growth factor and advanced retinopathy of prematurity

Objective To investigate the relationship between the gene polymorphism of vascular endothelial growth factor （VEGF） and the advanced retinopathy of prematurity （ROP）. Methods Retrievals of the literature in Cochrane Library, PubMed, EMBASE, CNKI, Wanfang Data, and Sino Med Database were conducted. The deadline of searches was December 30, 2013 and search words were vascular endothelial growth factor, retinopathy of prematurity, and polymorphism. References of important papers were retrospectively retrieved. Randomized controlled trials （RCTs） relevant to VEGF and advanced ROP were obtained for comparative study and the quality of papers was evaluated. The heterogeneity was estimated by the State 10.0 and the meta analysis was performed according to the results of estimation. Results A total of 9 papers （9 independent studies） with 1745 samples were selected to analyze. The funnel plot showed that there was no significant publication bias. The results of meta analysis indicated that mutations of three andes, i.e. 405 G→C （rs2010963）, -460 T→C （ rs833061）, and 936 C→T （ rs3025039）, were not relevant to patients with advanced ROP. Their OR values were 0.95 （ 95% CI： 0. 67 - 1.36, P = 0.79）, 1.20 （95% CI： 0. 89 - 1.61, P=0.23）, and 1.23（95% CI： 0. 43 -3.53, P=0.7）, respectively. Conclusion The polymorphism of 405 G→C （rs2010963）, -460 T→C （rs833061）, and 936 C→T （rs3025039） of VEGF gene are not relevant to the advanced ROP.