IgA肾病肾组织中CD71表达的分子特征

目的 阐明转铁蛋白受体(CD71)在IgA肾病(IgAN)肾组织中表达的分子构象特征及其与IgA分子构象特征之间的相互关系,探讨它在IgAN免疫发病机制中的作用.方法 170例肾活检患者根据临床表现和肾活检病理诊断分为原发性IgAN组(原发组)、非IgAN性IgA沉积患者组(继发组)、无IgA沉积肾病组(对照组).在激光共聚焦荧光显微镜下,运用免疫荧光双套色法标记的抗体观察四甲基罗丹明(TRITC)标记CD71和异硫氰荧光素(FITC)标记IgA在肾组织上的分子表达.结果 CD71在对照组患者的肾小球上呈微弱表达或不表达,在原发组和继发组呈不同程度的阳性表达,且在原发组呈阳性高表达.在激光共聚焦荧光显微镜下观察到CD71的表达与IgA表达呈共位.结论 CD71在IgAN患者肾小球上呈高表达,且与IgA分子呈共位状态,说明CD71参与了IgAN的发病过程,提示CD71在IgAN免疫发病机制中发挥着重要的作用.

Molecular features of CD71 expression in renal tissue in IgA nephropathy

Objective To study the molecular pathologic features of CD71 and the correlation between CD71 expression and IgA deposit in IgA nephropathy(IgAN),and to investigate the role of CD71 in pathogenesis of IgAN in order to direct the therapeutic strategies.Methods According to clinical manifestations and pathological diagnosis,170 cases of renal biopsy were divided into IgA deposition nephritis(primary group),non-IgAN with IgA deposition(secondary group)and no IgA deposited nephritis(control group).Immunofluorescence double-staining using TRITC-conjugated anti CD71 antibody and FITC-conjugated anti-human IgA antibody was performed to illustrate the expression of CD71 and IgA respectively by confocal microscopy.Results No CD71 expression was found in control group,while the CD71 was positively expressed in primary and secondary groups to various levels.The intensity of expression of CD71 in primary group was more intense than that in the secondary group.The expression of CD71 in the IgAN tissues correlated closely with the accumulation of IgA.CD71 and IgA were distributed in mesangial area and capillary lumina of glomeruli of IgAN(primary group).The study showed that CD71 expression and IgA deposition were co-localized under confocal fluorescence microscopy.Conclusion CD71 is highly expressed in the glomeruli of primary IgAN.The expression of CD71 in the IgAN tissues correlats closely with the accumulation of IgA.CD71 and IgA are co-localized under confocal fluorescence microscopy.The present study reveals that CD71 is one of IgA receptors and involves in the course of IgAN.The results suggests that CD71 may play an important role in immunopathogenesis of IgAN.