| 木鳖子素的分离与纯化 |
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| 引用本文： | 王秀琴,李檀,白宗利,贾天柱. 木鳖子素的分离与纯化[J]. 辽宁中医杂志, 2007, 34(1): 78-79 |
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| 作者姓名： | 王秀琴 李檀 白宗利 贾天柱 |
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| 作者单位： | 辽宁省血栓病中西医结合医疗中心,辽宁,沈阳,110101;沈阳市苏家屯区中医院,辽宁,沈阳,110101;辽宁中医药大学,辽宁,沈阳,110032 |
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| 摘 要： | 目的:分离纯化到单一组分的木鳖子素。方法:木鳖子脱脂粉用磷酸盐缓冲液提取,提取液用硫酸铵沉淀,沉淀用水溶解后先上CM Sepharose Fast F low阳离子交换凝胶柱然后上SephacrylTMS-200 H igh F low凝胶柱。结果:纯化到单一组分的木鳖子素,分子量为29000。结论:该方法快速、稳定。
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| 关 键 词： | 木鳖子素 CM Sepharose Fast Flow阳离子交换凝胶柱 SephacrylTMS-200 High Flow凝胶柱 分子量 |
| 文章编号： | 1000-1719(2007)01-0078-02 |
| 修稿时间： | 2006-04-06 |
Isolation and Purification of Cochinchinin |
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| WANG Xiu-qin,LI Tan,BAI Zong-li,JIA Tian-zhu. Isolation and Purification of Cochinchinin[J]. Liaoning Journal of Traditional Chinese Medicine, 2007, 34(1): 78-79 |
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| Authors: | WANG Xiu-qin LI Tan BAI Zong-li JIA Tian-zhu |
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| Affiliation: | 1. Liaoning Provincal Thrombas Disease Treating Center,Shenyang 110101 ,Liaoning, China ;2. Sufiatun District Hospital of TCM , Shenyang 110101, Liaoning , China ;3. Liaoning University of TCM , Shenyang 110032, Liaoning, China |
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| Abstract: | Cochinchinin was separated and purified from the seed extract of Momordica Cochinchinensis,Chinese herbal medicine mubiezi,by precipitation at 30—60%saturation of(NH_4)_2SO_4,CM Sepharose Fast Flow and Sephacryl~TM S-200 High Flow column chromatography.It proved to be homogeneous by SDS—PAGE with a molecular weight of 29000. |
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| Keywords: | Cochinchinin CM Sepharose Fast Flow SephacrylTM S - 200 High Flow molecular weight |
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