不同临床分期湿疹患者外周血中IL-7和IL-10水平变化及其临床意义

目的：检测不同临床分期湿疹患者外周血中白细胞介素7（IL-7）和白细胞介素10（IL-10）的水平，并阐明其临床意义。方法：选择90例湿疹患者，分为急性期湿疹组、亚急性期湿疹组和慢性期湿疹组，每组各30例；选择同期行健康体检的志愿者30人作为对照组。采用RT-PCR法检测各组研究对象外周血中IL-7和IL-10mRNA的相对表达水平，采用ELISA法测定各组研究对象外周血中IL-7和IL-10水平。计算各组研究对象湿疹面积和严重程度指数（EASI）评分。分析湿疹患者外周血中IL-7和IL-10水平与EASI评分的相关性。结果：与对照组比较，各期湿疹组患者外周血中IL-7和IL-10 mRNA相对表达水平明显升高（F=3.1，P=0.04；F=4.73，P=0.02），外周血中IL-7和IL-10水平也明显升高（F=6.23，P<0.01；F=5.34，P=0.01）。湿疹组患者外周血中IL-7和IL-10水平与其对应mRNA相对表达水平（r=0.95，P<0.01；r=0.94，P<0.01）及EASI评分均呈正相关关系（r=0.88，P<0.01；r=0.89，P<0.01），且均随着患者病情严重程度的加重而升高。结论：湿疹患者外周血中IL-7和IL-10水平明显高于健康体检者，且与病情严重程度呈正相关关系。细胞因子IL-7和IL-10可能参与了湿疹的病理过程，检测其表达水平有助于评估疾病的严重程度。

Changes of levels of IL-7 and IL-10 in peripheral blood of patients with different clinical stages of eczema and thier clinical significances

Objective: To detect the levels of interleukin-7 (IL-7) and IL-10 in peripheral blood of the patients with different clinical stages of eczama,and to elucidate their clinical significances. Methods: Ninety patients with eczema were selected,and divided into acute eczama group (n=30),subacute ecxama group (n=30),and chronic eczama group (n=30).Meanwhile,30 healthy people who went to hospital for physical examination during the same period were recruited as control group.The relative expression levels of cytokines IL-7 and IL-10 mRNA in peripheral blood of the subjects in various groups were detected by RT-PCR method.The levels of IL-7 and IL-10 in peripheral blood of the subjects in various groups were measured by ELISA method.The eczema area and severity index (EASI) scores of the subjects in various groups were detected.The relationships between IL-7 level, IL-10 level and EASI scores were analyzed. Results: Compared with control group,the relative expression levels IL-7 and IL-10 mRNA in peripheral blood of the patients with different clinical stages of eczenma were significantly increased (F=3.17,P=0.04; F=4.73,P=0.02);the expression levels of IL-7 and IL-10 in peripheral blood of the patients with different clinical stages of eczema were significantly increased (F=6.23,P<0.01; F=5.34,P=0.01).The levels of IL-7 and IL-10 were positively correlated with the corresponding mRNA relative expression levels in peripheral blood of the patients with different clinical stages of eczema (r=0.95,P<0.01; r=0.94,P<0.01) and EASI scores of the patients (r=0.88,P<0.01; r=0.89,P<0.01),which were increased along with the increasing of severity degree of the patients. Conclusion: The levels of IL-7 and IL-10 of the patients with eczema are significantly higher than those in the healthy controls,which are positively correlated with the severity of the disease. The cytokines IL-7 and IL-10 may be involved in the pathological process of eczema,and the detection of their experssion levels contributes to assess the severity of disease.