2010-2012年日照市手足口病病原学检测分析

目的了解日照市手足口病的病原学特征及影响阳性检出率的因素，为手足口病的早期诊断、标本检测及预防提供依据。方法共采集l010份手足口病病例标本，应用实时逆转录一聚合酶链反应（RT—PCR）法进行检测，并对结果进行分析。结果2010~2012年合计检测手足口病病例标本1010份，肠道病毒阳性的721份，阳性率为71．39％。其中，EV71病毒阳性330份，阳性率为32．67％；CoxAl6病毒阳性264份，阳性率为26．140；其他肠道病毒阳性127份，阳性率为12．57％。每年的肠道病毒阳性率分别为：2010年78．40％（127／162），2011年63．86％（357／559），2012年82．01％（237／289）。标本的肠道病毒阳性率：男性为72．48％，女性为69．25％（P〉O．05）；粪便标本为74．89％，咽拭子标本为35．56％（P〈O．01）；轻症者为68．83％，重症者为86．39％（P〈O．01）。阳性组平均年龄为（3．03±1．64）岁，阴性组为（3．30±2．07）岁（P〈O．05）。多因素非条件Logistic回归分析结果，标本类型和症状轻重是影响标本检测阳性率的影响因素，OR分别为5．305、0．352。结论标本类型和症状轻重为肠道病毒阳性率的影响因素，年龄可能对肠道病毒阳性率有影响。

Analysis on Etiological Testing of HFMD,Rizhao City, 2010-2012

Objective To understand the etiological features of HFMD and the influencing factors of sample positive rate, so as to provide the basis for early diagnosis,sample test,and prevention of HFMD. Methods A total of 1 010 samples of HFMD were collected,the samples were teated by real-time fluorescence quantitative RT-PCR, then the results were analyzed. Results A total of 1 010 samples of HFMD were tested during 2010-2012,721 were enterovirus positive,the average positive rate was 71.39%. Among them, the positive rate of EVT1, CoxA16 and other types of enterovirus was 32.67% ,26.14% and 12.57%. The positive rate of 2010,2011 and 2012 was 78.40%（127/162） ,63.86%（357/559） and 82.01 % （237/289）. The enterovirus-positive rates of samples were males 72.48% and females 69.25% （ P 〉0.05） ,dejec- tion 74.89% and throat swab 35.56% （ P 〈0.01）, mild cases 68.83% and severe cases 86.39 % （ P 〈0.01）. The average age of positive group was （3.03 ± 1.64）, negative group was （3.30± 2.07）（ P 〈O. 05）. Logistic multiple regression anal- ysis showed that sample type and symptom degree were the influencing factors to positive rate, the OR was 5. 305 and 0. 352. Conclusion Sample type and symptoms degree are the influencing factors of enterovirus-positive rate, age may affect the enterovirus positive rate.