Method to identify genomic targets of DNA binding proteins. |
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| Authors: | L Sompayrac and K J Danna |
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| Affiliation: | Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309-0347. |
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| Abstract: | We have devised a cyclical immunoprecipitation protocol that can be used to identify and clone a specific DNA sequence that is recognized by a DNA binding protein, even if that sequence is present in only one copy in the genome of a mammal. As an example, we have used this procedure to purify mouse genomic sequences to which the simian virus 40 tumor (T) antigen binds. |
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