糖皮质激素对哮喘模型大鼠肾阴阳虚证候演变的影响

目的：观察地塞米松干预哮喘模型大鼠的不同阶段（撤停前、撤停中、撤停后）对阴阳虚证候及下丘脑一垂体一肾上腺皮质（hypothalamic-pituitary-adrenal，HPA）轴的影响。方法：通过雾化吸入1％卵清白蛋白建立大鼠慢性哮喘模型。腹腔注射地塞米松模拟激素干预过程，观察不同阶段大鼠的症状表现，根据阴虚、阳虚证计量标准，判断阴虚、阳虚证候属性；酶联免疫吸附测定法检测血清皮质酮（corticosterone，CORT）含量，放射免疫测定法检测血浆促肾上腺皮质激素（adrenocorticotropic hormone，ACTH）、下丘脑匀浆液促肾上腺皮质激素释放激素（corticotropin—releasing hormone，CRH）及血浆骨钙素（bone Gla—protein，BGP）含量。结果：激素干预组大鼠于激素撤停前阶段属阴虚证，撤停中阶段属阴亏、阳虚证，撤停后阶段属阴阳两虚证。激素撤停前阶段及撤停中阶段，对HPA轴（CORT、ACTH、CRH含量）有显著抑制作用，激素撤停后HPA轴的功能有所恢复；激素应用的各个阶段，均存在BGP的明显抑制。结论：激素干预哮喘模型大鼠在激素应用的不同阶段具有不同的证候特点，激素干预后动物呈现明显的肾虚表现。

Effects of a glucocorticoid on development of kidney deficiency syndrome in a rat model of asthma

Objective： To observe the effects of dexamethasone （DXM） on changes of syndrome and hypothalamicpituitary-adrenal （HPA） axis of asthmatic rats at different stages of intervention （before withdrawal, during withdrawal and after withdrawal）,Methods： Asthma was induced in rats by inhalation of 1% ovalbumin. Rats in DXM group were treated with different concentrations of DXM by intra-abdominal injection. At the different stages of DXM treatment （before withdrawal, during withdrawal and after withdrawal）, symptoms of the rats were observed to differentiate the syndromes according to yin-deficiency and yang-deficiency measurement standard. Content of serum corticosterone （CORT） was detected by enzyme-linked immunosorbent assay, and contents of plasma adrenocorticotropic hormone （ACTH） and bone Gla-protein （BGP）, and content of corticotropin-releasing hormone （CRH） in hypothalamic homogenate were detected by radioimmunoassay.Results： Rats in the DXM group were differentiated as yin-deficiency syndrome before the stage of hormone withdrawal. During the period of hormone withdrawal and after the withdrawal, syndromes of rats in the DXM group accorded with the standards of yin-and yang-deficiency. During the period of treatment with DXM, contents of CORT, ACTH and CRH were significantly decreased, but recovered after DXM withdrawal. BGP level in the DXM group was lower than those in the normal group and the model group during all the stages. Conclusion： Asthmatic rats treated with DXM have different syndrome characteristics in different stages. Kidney deficiency will be caused by DXM treatment.