郧阳木瓜中齐墩果酸和熊果酸的含量测定

目的：测定郧阳木瓜有效成分齐墩果酸和熊果酸的含量。方法：采用反相高效液相色谱法（RP—HPLC），Agilent HC—C18（250mm×4．6mm，5μm）色谱柱，以乙腈-0．01％磷酸溶液（80：20）为流动相，流速0．8mL／min，柱温18℃，检测波长210nm。结果：齐墩果酸和熊果酸分别在0．84～16．80μg／mL和1．21～24．20μg／mL范围内有良好的线性关系，平均加样回收率分别为98．4％、98．2％，RSD分别为0．78％、0．98％。郧阳木瓜中齐墩果酸和熊果酸的含量分别为0．41mg／g和0．63mg／g。结论：湖北郧县地区所产郧阳木瓜中齐墩果酸和熊果酸含量较高，具有一定的食疗价值。

Determination of Olennolic Acid and Ursolic Acid in Fructus Chaenomelis of Yunyang

Objective： To establish the RP-HPLC method for the determination of olennolie acid and ursolie acid in Fruetus Chaenomelis of Yunyang. Methods： The Agilent HC-C18（250 mm×4.6 mm, 5 μm） column was used; the mobile phase consisted of acetonitrile ： 0.01% H3PO4（80 ： 20） ; the flow rate was 0. 8 mL/min; the column temperature was 18 ℃ ; the detecting wavelength was at 210 nm. Results： The calibration curve was linear within a range of 0.84 - 16.80 μg/mL and 1.21 - 24.20 μg/mL; the average recovery of this method was 98.4% and 98.2% ; the RSD was 0.78% and 0.98%, respectively. Conclusions： The contents of olennolic acid and ursolic acid in fructus chaenomelis of Yunyang have better dietary therapy value.