子宫颈鳞癌组织中基质金属蛋白酶MMP-2和MMP-9的表达及其意义

目的探讨基质金属蛋白酶MMP-2和MMP-9与宫颈鳞癌的发生、发展及转移的关系.方法免疫组化S-P法测定MMP-2和MMP-9表达及分布;明胶酶谱法测定活性型MMP-2及MMP-9蛋白的含量;RT-PCR技术检测MMP-2及MMP-9 mRNA表达水平.结果宫颈癌组织中MMP-2及MMP-9的阳性表达率为77.78%和66.67%,明显高于在CIN(10%及20%)及正常宫颈组织中表达率.淋巴转移组MMP-2及MMP-9的阳性率均显著高于非淋巴转移组,P<0.05.在宫颈鳞癌组织中MMP-2及MMP-9酶活性显著高于CIN及正常宫颈组织,P<0.01.MMP-2及MMP-9 mRNA在宫颈鳞癌中的表达量亦显著高于在CIN及正常宫颈组织的表达量,P<0.01.结论MMP-2及MMP-9与宫颈鳞癌的发生、发展及转移有关,MMP-2及MMP-9的增高可作为判断宫颈鳞癌具有转移倾向的临床参考指标.

Expression of Matrix Metalloproteinase in Cervical Squamous Cell Carcinoma Tissue and Its Significance

Objective To investigate the relationship between the expression of matrix metalloproteinases (MMP 2,MMP 9) and tumorigenesis,development and metastasis of cervical squamous cell carcinoma.Methods The expression and distribution of MMP 2,MMP 9 proteins were detected by using the immunohistochemical S P method.Their active proteins and mRNA were analyzed by the gelatin zymography and RT PCR respectively.Results MMP 2 and MMP 9 proteins majorly localized in the plasma,minor in the membrane.The positive expression rates of MMP 2 and MMP 9 were 77 78 and 66 67% respectively,in the carcinoma tissues.The rate in the subgroup with lymphatic metastasis was significantly higher than that in the group without metastasis, P <0 05.The presence of MMP 2 and MMP 9 proteins showed no relationship between pathologic grade,clinical stage and the size of the carcinoma.In CIN,the positive rates for this two proteins were 10% and 20% respectively.However,they were absent in normal tissues.The activities of MMP 2 and MMP 9 in carcinoma tissues,which analyzed by the gelatin zymography were statistically higher than those in CIN and normal cervices, P <0 01.MMP 2 mRNA and MMP 9 mRNA were significantly higher in carcinoma than those in CIN and normal cervices, P <0 01.Conclusions MMP 2 and MMP 9 correlate well with the occurrence,invasion and metastasis of cervical squamous cell carcinoma.The two indicators can be used to identify the metastasis trend of cervical carcinoma in clinic.