离子色谱法测定小儿止咳糖浆中氯化铵的含量

目的 建立离子色谱法测定小儿止咳糖浆中氯化铵含量的方法。方法 离子色谱法采用IonPac^TM AS11-HC阴离子交换色谱柱(2.0 mm×250 mm)与IonPac^TM AG11-HC保护柱(2.0 mm×50 mm)，检测器为抑制电导检测器，电导检测池温度为35℃，柱温为30℃，进样量为25 μL，以10 mmol·L^–1氢氧化钾溶液为流动相，流速为0.5 mL·min^–1。结果 氯离子浓度为10~400μg·mL^–1时，峰面积与其浓度呈良好的线性关系(r=1.000 0，n=6);平均回收率为100.57%(RSD=0.41%，n=6)。结论 该方法专属性强、准确度高，可用于小儿止咳糖浆中氯化铵的含量测定。

Determination of Ammonium Chloride in Xiao'er Zhike Syrup by Ion Chromatography

OBJECTIVE To establish an ion chromatography method for determination of ammonium chloride in Xiao'er Zhike syrup. METHODS The chromatographic separation was performed on an IonPac^TMAS11-HC anion exchange column (2.0 mm×250 mm) and IonPac^TM AG11-HC guard column(2.0 mm×50 mm), using suppressed conductivity detector, the temperature of the detection tank and the column were set at 35℃ and 30℃ respectively, the injection volume was 25 μL, an isocratic eluent of 10 mmol·L^-1 KOH was chosen as mobile phase and the flow rate was 0.5 mL·min^-1. RESULTS A good linear relationship between the peak area and its concentration was observed within the range of 10-400 μg·mL^-1(r=1.000 0, n=6); The average recovery was 100.57%(RSD=0.41%, n=6).CONCLUSION The method is specific and accurate, and can be used for determination of ammonium chloride in Xiao'er Zhike syrup.