| 高效液相色谱法测定大风丸中蛇床子素的含量 |
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| 引用本文： | 蔡俊安,王粉. 高效液相色谱法测定大风丸中蛇床子素的含量[J]. 中国中医药信息杂志, 2009, 16(8): 47-48 |
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| 作者姓名： | 蔡俊安 王粉 |
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| 作者单位： | 河南百年康鑫药业有限公司,河南,郸城,477150 |
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| 摘 要： | 目的建立大风丸中蛇床子素含量的测定方法。方法采用高效液相色谱法，Diamonsil ODS1 C18柱，流动相为甲醇-0．1mol／L硫酸铵溶液（70：30），用硫酸溶液（1→5）调pH为2．5，流速为1．0mL／min，检测波长322nm。结果蛇床子素在0．101-0．808μg范围内呈良好线性，回归方程为r=9．964406×10^-3X-1．355684×10^-3，r=0．9995，平均加样回收率为98．4％，RSD=1．40％（n=6）。结论本方法简便、准确，专属性强，测定结果重复性好，是大风丸中蛇床子素定量分析的有效方法。
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| 关 键 词： | 大风丸 蛇床子素 高效液相色谱法 含量测定 |
Determination of Fructus Cnidii in Dafeng Wan by HPLC |
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| CAI Jun-an,WANG Fen. Determination of Fructus Cnidii in Dafeng Wan by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2009, 16(8): 47-48 |
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| Authors: | CAI Jun-an WANG Fen |
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| Affiliation: | (Henan BainianKangxin Pharmaceutical Co. Ltd, Dancheng 477150, China) |
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| Abstract: | Objective To establish the method for determining the content of Fructus Cnidii in Dafeng wan by HPLC. Methods Diamonsil ODS1 C18 column was used with CH3OH-0.1 mol/L （NH4）2SO4 （70 ： 30） [adjust pH to 2.5 by sulphuric acid solution {145}] as the mobile phase. The detection wavelength was set at 322 nm and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.101-0.808 μg for the Fructus Cnidii and linear equation was Y=9.964406× 10^-3X-1.355684X 10^-1, r=0.999 5. The average recovery was 98.4% and RSD was 1.40% （n =6）. Conclusion This method was simple and accurate, with good specificity and repeatability, which provides scientific quantitative analysis method of Fructus Cnidii in Dafeng wan. |
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| Keywords: | Dafengwan Fructus Cnidii HPLC content determination |
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