微板法检测蚊虫体内乙酰胆碱酯酶活性研究

目的：检测不同敏感品系（Ｓ）蚊虫和室内选育的抗性品系淡色库蚊体内乙酰胆碱酯酶活性。方法：微滴定板法。结果：４种Ｓ品系蚊虫中，以中华按蚊体内ＡｃｈＥ水平最高；３种抗性品系蚊虫中，抗残杀威品系的ＡｃｈＥ活性〉抗氯氰区酯品系〉抗ＤＤＶＰ品系，且后者一Ｓ品系差异无显著性。蚊虫体内ＡｃｈＥ活性随发育而逐步增高，以不同浓度残杀威作抑制剂，测定室内不同品系淡色库蚊个体的ＡｃｈＥ活性，表明蚊虫ＡｃｈＥ活性的抑制率

Microplate Test for the Determination of Acetylcholinesterase in Vector Mosquitoes

Microplate test was employed for the detection of acetylcholinesterase(AchE) level in susceptible mosquitoes and resistant strains of Culex pipiens pallens .Among 4 species of susceptible mosquitoes, Anopheles sinensis produced the highest level of AchE. The AchE level varied in different resistant strains of Cx. pipiens pallens , with anti propoxur strain the highest level,foollwed by anti-cypermethrin strain and with anti-DDVP strain the lowest. The AchE activity increased with the development of mosquito. Using propoxur(at 0.01mmol/L) as the inhibitor , the inhibition rate of AchE was highest in susceptible strain,followed by anti-cypermethrin strain ,anti-DDVP strain and anti-propoxur strain ,in descending order.The sensitivity of AchE was higher in susceptible strain than in resistant strains.