Na+-independent,nifedipine-resistant rat afferent arteriolar Ca2+ responses to noradrenaline: possible role of TRPC channels |
| |
Authors: | M. Salomonsson T. H. Braunstein N.-H. Holstein-Rathlou L. J. Jensen |
| |
Affiliation: | 1. Division of Renal and Vascular Research, Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark;2. Present address: Danish National Research Foundation Center for Cardiac Arrhythmia, Department of Biomedical Sciences, University of Copenhagen, Blegdamsvej 3, Building 10.5, DK-2200 Copenhagen N, Denmark.;3. Present address: Section for Biochemistry, Physiology and Nutrition, Department of Basic Animal and Veterinary Sciences, University of Copenhagen, DK-1870 Frederiksberg C, Denmark. |
| |
Abstract: | Aim: In rat afferent arterioles we investigated the role of Na+ entry in noradrenaline (NA)-induced depolarization and voltage-dependent Ca2+ entry together with the importance of the transient receptor potential channel (TRPC) subfamily for non-voltage-dependent Ca2+ entry. Methods: R 340/380 Fura-2 fluorescence was used as an index for intracellular free Ca2+ concentration ([Ca2+]i). Immunofluorescence detected the expression of TRPC channels. Results: TRPC 1, 3 and 6 were expressed in afferent arteriolar vascular smooth muscle cells. Under extracellular Na+-free (0 Na) conditions, the plateau response to NA was 115% of the baseline R340/380 (control response 123%). However, as the R340/380 baseline increased (7%) after 0 Na the plateau reached the same level as during control conditions. Similar responses were obtained after blockade of the Na+/Ca2+ exchanger. The L-type blocker nifedipine reduced the plateau response to NA both under control (from 134% to 116% of baseline) and 0 Na conditions (from 112% to 103% of baseline). In the presence of nifedipine, the putative TRPC channel blockers SKF 96365 (30 μm ) and Gd3+ (100 μm ) further reduced the plateau Ca2+ responses to NA (from 117% to 102% and from 117% to 110% respectively). Conclusion: We found that Na+ is not crucial for the NA-induced depolarization that mediates Ca2+ entry via L-type channels. In addition, the results are consistent with the idea that TRPC1/3/6 Ca2+-permeable cation channels expressed in afferent arteriolar smooth muscle cells mediate Ca2+ entry during NA stimulation. |
| |
Keywords: | adrenoceptors afferent arteriole renal haemodynamics ROC VSMC |
|
|