Evaluation of three methods for high throughput extraction of DNA from challenging fish tissues |
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Authors: | Helge Meissner Svein-Erik Fevolden Per-Arne Amundsen Kim Præbel |
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Affiliation: | 1. Department of Arctic and Marine Biology, University of Troms?, 9037, Troms?, Norway 2. Norwegian Forest and Landscape Institute, 1431, ?s, Norway
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Abstract: | Three methods for extracting DNA were tested on otoliths, scales, fins, and gill tissue from European whitefish [Coregonus lavaratus (L.)]. The aim was to find time-efficient and affordable ways to simultaneously extract DNA suitable for conservation genetic studies from a large number of samples and different tissues. A rapid low-cost method led to 97 % success of microsatellite amplification in otoliths and 100 % in scales. High amplification success was achieved with fin (97 %) and gill (99 %) tissue using a salt lysis-based protocol. A commercial extraction kit delivered good results with all tissues. The findings are useful for conservation genetic studies using both contemporary and archived samples. |
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