甲基纤维素体外分离骨髓移植物中红细胞的效果

“ABO”血型不合的骨髓移植和一部分自体骨髓移植 ,需分离骨髓移植物中的红细胞。本研究观察了用甲基纤维素分离移植物中红细胞的效果。用生理盐水配制的 0 .5 %甲基纤维素 (MC)与采集的骨髓移植物按一定比例混匀 ,静置 ,然后沉降红细胞。对 1 8份骨髓移植物分离的结果显示 ,单个核细胞和CD34+ 细胞回收率分别达(83 .8± 5 .2 ) %和 (90 .3± 7.2 ) % ,CFU GM产率为 (60 .8± 2 2 .4) / 2× 1 0 5个单个核细胞 ;红细胞残留率仅为 (4.3±1 5) %。结论 :此方法可用于骨髓移植

The Effect of Separating Red Blood Cells from Bone Marrow Graft In Vitro by Methylcellulose

It has need to separate red blood cells (RBC) from marrow graft in ABO group unmatched BMT and auto-BMT with purging tumor cells, the separating effect of methylcellulose was observed. The mixture of 0.5% methylcellulose and bone marrow was laid up in an open transfusion system, and then sedimentation of RBC was performed in the transfusion tube. The separating results of 18 marrow grafts showed that the recovery rates of mononuclear cells and CD34(+) cells were (83.8 +/- 55.2)% and (90.3 +/- 7.2)%, respectively. RBC residual rate was (4.3 +/- 1.5)%. The yield of CFU-GM was (60.8 +/- 22.4)/2 x 10(5) MNC, and there was no difference to (69.8 +/- 23.4)/2 x 10(5) MNC] yielded from same marrow samples, separated by Ficoll-Hypaque separation. It is concluded that this method could be used for bone marrow transplantation.